RNA干扰对XWLC-05肺癌细胞水通道蛋白3表达的影响  

Knockdown the expression of aquaporin-3(AQP3) in XWLC-05 human lung cancer cells by small interfering RNA

作　　者：张璟[1] 黄云超[2] 周永春[2] 陈琬玲 

机构地区：[1]昆明医学院第一附属医院,昆明650000 [2]昆明医学院第三附属医院 [3]武警云南省总队医院

出　　处：《山东医药》2011年第9期25-27,共3页Shandong Medical Journal

基　　金：云南省社会发展科技计划-国际合作专项基金项目(2008CA030);昆明医学院博士研究生创新基金项目(KM2008D06)

摘　　要：目的构建靶向水通道蛋白3(AQP3)的siRNA表达载体,探讨其对XWLC-05肺癌细胞系AQP3表达的影响。方法构建AQP3特异性siRNA表达载体和对照组表达载体,以脂质体法转染XWLC-05肺癌细胞,用RT-PCR检测AQP3 mRNA的表达,用Western blot方法检测APQ3蛋白的表达。结果成功构建靶向AQP3的siRNA表达载体1和2,分别命名为pAQP3-siRNA1、pAQP3-siRNA2。转染48 h后pAQP3-siRNA1和pAQP3-siRNA2转染组AQP3 mRNA的表达量分别是对照组的65%和79%,组间比较有统计学差异(P<0.05);pAQP3-siRNA1和pAQP3-siRNA2转染后的蛋白表达量分别是对照组的53%和73%,pAQP3-siRNA1干扰效果明显高于pAQP3-siR-NA2(P<0.05)。结论 AQP3特异性siRNA能够有效地抑制XWLC-05肺癌细胞系AQP3的表达。Objective To construct the siRNA expression vector targeting AQP3 and to evaluate its ability to down-regulate the expression of AQP3 in XWLC-05 human lung cancer cells. Methods Two siRNA expression vectors, pAQP3- siRNA1, pAQP3-siRNA2 targeting AQP3, were constructed. After pAQP3-siRNA1 and pAQP3-siRNA2 were transfected into XWLC-05 lung cancer cells, the levels of mRNA and protein expressions in XWLC-O5 human lung cancer ceils were determined by RT-PCR and Western blot analysis respectively. Results Two siRNA expression vectors targeting AQP3 were constructed successfully, named pAQP3-siRNA1 and pAQP3-siRNA2 respectively. Forty-eight hours after transfection, level of AQP3-mRNA was decreased significantly in AQP3-aiRNA1 XWLC-05 ceils （65% of that in control group） compared with AQP3-siRNA2 XWLC-05 cells （79% of that in control group ） （P 〈 0.05 ）. The level of protein expression of AQP3 in AQP3-siRNA1 XWLC-05 cells decreased significantly （53% of that in control group ） compared with AQP3- siRNA2 XWLC-05 cells （73% of that in control group） （ P 〈 0.05）. Conclusions AQP3 specific siRNA can significantly and specifically down-regulate the expression of AQP3 in XWLC-05 cells.

关 键 词：肺肿瘤 水通道蛋白3 小干扰RNA 

分 类 号：R734[医药卫生—肿瘤]