小鼠EDNRB基因启动子生物信息学分析  

Bioinformatics Analysis of Mouse EDNRB Gene Promoter Regions

作　　者：张万里[1] 李伟[1] 吴川清[1] 林学科[1] 李航[1] 王国斌[1] 陶凯雄[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022

出　　处：《生物信息学》2011年第1期72-75,81,共5页Chinese Journal of Bioinformatics

基　　金：国家自然科学基金资助项目(30872473)

摘　　要：EDNRB/EDN3/ECE-1信号传导通路在胚胎发育期神经嵴细胞分化、迁移、发育为神经节细胞的过程中起到关键作用。采用进化足迹法,利用生物信息学在线软件分析先天性巨结肠相关EDNRB基因启动子序列,结果显示:小鼠EDNRB基因定位于14号染色体,编码442个氨基酸,分子量为:49430Da,转录起始点300bp左右可能为启动子区域,启动子区域包含长度为1473bp的CpG岛,存在两段保守序列,人类和小鼠保守区域内含有33个共同的转录因子结合位点。EDNRB/EDN3/ECE-1 signaling pathway plays an important role in the differentiation and migration of neural crest cells to form ganglion cells during embryonic development.Online bioinformatics tools and phylogenetic foot-printing were used to analyze Hirschsprung’s disease associated EDNRB gene’s promoter.The results showed that mouse EDNRB gene was mapped to chromosome 14,which encoded a protein consisting of 442 amino acids with a molecular weight 49430Da.300bp around transcriptional start site might be promoter regions,which included a CpG island with a length of 1473bp.The promoter regions had two conserved sequences,which contained 33 same transcriptional factor binding sites.

关 键 词：EDNRB 启动子 生物信息学 转录因子 

分 类 号：Q518.2[生物学—生物化学]