扶正合剂结合腹腔化疗对小鼠恶性腹水细胞核仁组成区相关的嗜银蛋白的干预作用  

Intervention of AgNOR on malignant ascites with Fuzheng mixture combined with intraperitoneal chemotherapy

作　　者：贾文娟[1] 田菲[2] 蔡刚强[3] 

机构地区：[1]天津市和平区新华职工大学中医科,天津300040 [2]天津中医药大学第一附属医院肿瘤科,天津300193 [3]天津中医药大学第一附属医院检验科,天津300193

出　　处：《河北中医》2011年第1期110-111,158,共3页Hebei Journal of Traditional Chinese Medicine

摘　　要：目的研究扶正合剂结合腹腔化疗对小鼠恶性腹水细胞核仁组成区相关的嗜银蛋白(Ag-NOR)的干预作用。方法昆明种小鼠40只,造模后随机分为4组,即空白组、顺铂组、扶正组、结合组。其中空白组予0.9%氯化钠注射液灌胃及腹腔注射,顺铂组予顺铂溶液腹腔注射,扶正组予扶正合剂灌胃,结合组予扶正合剂灌胃及顺铂溶液腹腔注射。给药12 d后抽取腹水,嗜银染色观察AgNOR颗粒形态、分布、颗粒计数及核仁银染区面积/核面积(I.S)值。结果颗粒数及I.S值方面,顺铂组、结合组与空白组及扶正组比较差异均有统计学意义(P<0.05),结合组与顺铂组比较差异无统计学意义(P>0.05)。在形态分布方面,4组间比较差异无统计学意义(P>0.05)。结论扶正合剂结合腹腔化疗可以调节小鼠腹水细胞AgNOR颗粒数及I.S值,从而抑制肿瘤细胞增殖,但对AgNOR颗粒形态分布无显著影响。Objective To study the intervention of AgNOR on malignant ascites with Fuzheng mixture combined with intraperitoneal chemotherapy.Methods 40 mice of species of Kunming were randomly divided into 4 groups.The groups are divided into blank group,DDP group,Fuzheng group and combination group.Mice in blank group,DDP group,Fuzheng group and combination group received Sodium chloride,cisplatin,Fuzheng mixture and combination of Fuzheng mixture and intraperitoneal chemotherapy,respectively.Morphous,distribution and particle counting of AgNOR granules and area of silver staining nucleolar/nuclear area（I.S） were observed by Silver staining.Results There was significant difference between DDP group,combination group,blank group and Fuzheng group on particle shape,particle counting and I.S（P0.05）.There was no difference between DDP group and combination group（P0.05）.There was no difference between DDP group,combination group,blank group and Fuzheng group on distribution of shape（P0.05）.Conclusion Fuzheng mixture combined with intraperitoneal chemotherapy can adjust the number of AgNOR granules and I.S in the same simple remission rate,and inhibit tumor cell proliferation.

关 键 词：腹腔 药物疗法 核仁组成区 腹水 恶性 中药疗法 

分 类 号：R-332[医药卫生] R442.5