苦菊提取物的抗氧化活性研究  

Antioxidant Activity of Extract from Cichorium endivia L.

作　　者：陈超杰[1] 秦海林[2,3] 邓安珺[2,3] 王爱平[1] 

机构地区：[1]新药安全评价研究中心中国医学科学院/北京协和医学院,北京100050 [2]中草药物质基础与资源利用教育部重点实验室中国医学科学院 [3]北京协和医学院,北京100050

出　　处：《食品与药品》2011年第3期93-96,共4页Food and Drug

基　　金："重大新药创制"科技重大专项课题(2008ZX09305-001)

摘　　要：目的研究苦菊提取物的体外抗氧化作用。方法苦菊经95%乙醇加热回流、石油醚脱脂、乙酸乙酯提取和大孔吸附树脂乙醇洗脱,取60%乙醇的洗脱液挥干后得到提取物。通过二苯代苦味酰自由基(DPPH·)和超氧阴离子(O2-)清除能力的检测,及2,2’-偶氮-双-(2-脒基丙烷)氯化二氢(AAPH)诱导的红细胞溶血模型的测试,总体评价该提取物的体外抗氧化活性。结果该提取物能有效清除DPPH·和O2-·,其清除能力呈浓度依赖性,且清除O2-·的能力比阳性对照药维生素C更佳。同时,该提取物能有效防止AAPH诱导的兔红细胞溶血,提示具有预防机体细胞组织氧化应激损伤的作用。结论苦菊提取物具有很好的抗氧化活性,为其在食品和药品的抗氧化剂应用提供参考。Objective To study the antioxidant activity of extract from Cichorium endivia L..Methods C.endivia L.was refluxed with 95% ethanol and defatted with petroleum ether,and then was extracted by ethyl acetate and purified by macroporous resin successively,with ethanol as eluent.After elution with 60% ethanol,the eluate was evaporated,and the residue of the extract was obtained.The antioxidant activity of the extract was measured by 2,2-diphenyl-1-picrylhydrazyl radical（DPPH？） and superoxide anion（O2-·） scavenging capacity assays,and by the test based on haemolysis model induced by 2,2’-azobis（2-amidinopropane） dihydrochloride（AAPH）.Results The extract showed the capacity of scavenging radicals of DPP· and O2-·,which was dependent on the concentration of it,and its power to scavenge O2-· was stronger than that of the reference vitamin C.Meanwhile,the extract inhibited haemolysis of rabbit erythrocytes induced by AAPH effectively,indicating that the extract was able to protect the cells and tissues of organism from injury induced by oxidative stress.Conclusion The excellent antioxidant capacity of etract from Cichorium endivia L.provides reference for its application in food and drug.

关 键 词：苦菊 二苯代苦味酰自由基 超氧阴离子 红细胞溶血 抗氧化活性 

分 类 号：R282.7[医药卫生—中药学]