397例急性白血病细胞遗传学与FAB分型相关性研究  

Study on Cytogenetic Changes with Relation to FAB Classification in 397 Patients with Acute Leukemias

作　　者：霍菲菲[1] 刘欣[1] 孙自敏[1] 朱薇波[1] 郑昌成[1] 汪健 邬志伟[3] 

机构地区：[1]安徽医科大学附属省立医院血液科,安徽合肥230001 [2]安徽医科大学附属省立医院血液病研究室,安徽合肥230001 [3]安徽医科大学附属省立医院中心实验室,安徽合肥230001

出　　处：《中国实验血液学杂志》2011年第1期6-10,共5页Journal of Experimental Hematology

摘　　要：本研究探讨急性白血病的细胞遗传学改变,了解染色体核型异常与急性白血病FAB分型的关系及其对预后因素的影响。397例急性白血病患者于治疗前抽取骨髓标本,采用短期细胞培养法制备染色体标本,应用G显带技术进行染色体核型分析。结果显示,397例急性白血病中78例无分裂相,319例可供染色体核型分析的患者中染色体核型异常175例(占54.9%)。在急性淋巴细胞白血病(ALL)、急性髓系白血病(AML)和急性混合细胞白血病(AMLL)3种类型的白血病患者中,染色体核型异常分别为33/120例(占27.5%)、129/252例(占51.2%)和13/25例(占52.0%)。超二倍体41例(占23.4%),亚二倍体22例(占12.5%),正常二倍体112例(占64.0%)。AML染色体核型异常中与FAB分型相关的特异性染色体重排74/129例(占57.4%)。结论:大约55%左右的急性白血病存在克隆性染色体异常,一些特异性染色体异常改变是急性白血病的细胞遗传学特征,与急性白血病的FAB分型有明显相关性,染色体检查和分子遗传学方法相结合,对于白血病的诊断、分型、治疗和预后都具有重要意义。The purpose of study was to investigate the cytogenetic abnormality of acute leukemias（AL）,to analyze the relationship in the chromosomal abnormality and the AL FAB types,and to explore the impact of the chromosomal abnormalities on the prognostic factors of AL.The chromosome karyotypes of 397 patients with AL were analyzed by means of bone marrow short-term culture and G banding technique.The results showed that in 319 out of 397 patients,the chromosome karyotypes could be analyzed,and the chromosomal abnormality occurred in 175 patients（54.9%）.In the patients with acute lymphocytic leukemia（ALL）,acute myelogenous leukemia（AML） and acute mixed-lineage leukemia（AMLL）,the chromosomal abnormality occurred respectively in 33 of 120 patients（27.5%）,129 of 252 patients（51.2%）and 13 of 25 patients（52.0%）.Hyper-diploids,hypo-diploids and diploids occurred in 41 of 175 patients（23.4%）,22 of 175 patients（12.5%）,and 112 of 175 patients（64.0%） respectively.In patients with AML the FAB type-associated chromosomal abnormality occurred in 69 of 129 patients（53.5%）.It is concluded that chromosomal abnormalities exist in about 55% AL patients.Some special chromosomal abnormalities are cytogenetic characteristics of AL,and obviously correlated with AL FAB types,the combination of chromosomal detection with cytogenetics is useful for the diagnosis of AL,and the evaluation of therapeutic effects and prognosis.

关 键 词：急性白血病 染色体 FAB分型 

分 类 号：R733.71[医药卫生—肿瘤]