白血病K562、K562/A02细胞中mthfr、dpyd基因单核苷酸多态性的检测  

Detection of Single Nucleotide Polymorphisms of mthfr and dpyd Genes in Leukemia Cell Lines K562 and K562/A02

作　　者：张文静[1] 陈宝安[1] 程坚[1] 鲍文[1] 仲悦娇[1] 高峰[1] 夏国华[1] 张孝平[1] 许佩佩[1] 彭苗新[1] 

机构地区：[1]东南大学医学院附属中大医院血液科,江苏南京210009

出　　处：《中国实验血液学杂志》2011年第1期11-14,共4页Journal of Experimental Hematology

基　　金：国家自然基金(编号30872970;30740062);高等学校博士学科点专项科研基金资助(编号20070286042)

摘　　要：本研究旨在检测白血病细胞株及其耐药株中两种药物代谢相关基因的单核苷酸多态性。培养白血病细胞株K562及其耐药株K562/A02,采用QIAamp DNA Blood Minikit试剂盒提取基因组DNA,设计引物,应用PCR技术扩增相应目的片段;采用基于基质辅助激光解吸电离飞行时间质谱技术检测mthfr基因rs1801131、rs1801133、rs2274976及dpyd基因rs1801159、rs1801160、rs17376848的单核苷酸多态性。结果表明,在K562和K562/A02两种细胞中,mthfr基因rs1801131基因型均为CA、rs1801133均为CC、rs2274976均为GG;dpyd基因rs1801159基因型均为GG、rs1801160均为GG、rs17376848均为AA。结论:mthfr、dpyd基因上述位点在K562和K562/A02两种细胞中基因表达无差别。This study was purposed to detect single nucleotide polymorphisms（SNP） of 2 pharmacokinetics-related genes in K562 and K562/A02 cell lines.Leukemia cell line K562 and its resistant line K562/A02 were cultured,the genomic DNA was isolated by QIAamp DNA Blood Mini kit,primers were designed,the related DNA fragments were amplified by PCR.The SNP genotyping of mthfr gene rs1801131,rs1801133 and rs2274976 and dpyd gene rs1801159,rs1801160 and rs17376848 was performed by means of matrix assisted laser desorption ionization-time of flight mass spectrometry method（MALDI-TOFMS）.The results showed that the genotype of mthfr gene locus 1801131 was AC,rs1801133 was CC,rs2274976 was GG,genotype of dpyd gene locus 1801159 was GG,rs1801160 was GG,rs17376848 was AA in both K562 and K562/A02 cell lines.It is concluded that the above-mentioned loci of mthfr and dpyd genes in K562 and K562/A02 cell lines are not expressed differently.

关 键 词：K562 K562/A02 MTHFR基因 dpyd基因 单核苷酸多态性 

分 类 号：R733.7[医药卫生—肿瘤] Q786[医药卫生—临床医学]