多发性骨髓瘤患者骨髓间充质干细胞趋化相关因子基因表达异常  

Chemotaxis-related Factors are Expressed Abnormally in Bone Marrow Mesenchymal Stem Cells of Multiple Myeloma Patients

作　　者：胡慧瑾[1] 陆化[1] 费小明[2] 李俊霞[1] 李建勇[1] 

机构地区：[1]南京医科大学第一附属医院血液科,江苏南京210029 [2]江苏大学附属医院血液科,江苏镇江212001

出　　处：《中国实验血液学杂志》2011年第1期59-63,共5页Journal of Experimental Hematology

基　　金：江苏省自然基金资助(BK2008236);江苏省高校自然科学基础研究面上项目资助(07KJB320074)

摘　　要：本研究探讨多发性骨髓瘤(MM)患者骨髓间充质干细胞(MSC)趋化相关因子HGF、SDF-1、MCP-1和IL-8的基因表达水平及临床意义。应用实时定量RT-PCR方法检测20例初发多发性骨髓瘤患者骨髓间充质干细胞HGF、SDF-1、MCP-1和IL-8的表达水平。结果显示,HGF mRNA在MMMSC的表达明显高于对照组,具有统计学意义(p<0.01),SDF-1 mRNA的表达较对照组低(p<0.05),MCP-1和IL-8 mRNA的表达在两组间无统计学差异(p>0.05)。结论:MM患者骨髓MSC均表达HGF、SDF-1、MCP-1和IL-8,MM患者骨髓微环境中MSC趋化相关因子表达存在异常。This study was aimed to investigate the mRNA expression levels of hepatocyte growth factor（HGF）,stromal cell-derived factor-1（SDF-1）,monocyte chemotactic protein-1（MCP-1） and interleukin-8（IL-8）in bone marrow mesenchymal stem cells（MSC） from multiple myeloma（MM） patients.The mRNA expression levels of HGF,SDF-1,MCP-1 and IL-8 in bone marrow MSC from 20 newly diagnosed MM patients were detected by real time quantitative RT-PCR and were compared with that in 9 controls.The results indicated that the mean mRNA expression level of HGF was up-regulated in MM patients,as compared with controls（p0.01）.However,the mean mRNA expression level of SDF-1 mRNA was down-regulated in MM patients,as compared with controls（p0.05）.There was no significant difference in the mRNA expression levels of MCP-1 and IL-8 between MM and control cohorts（p0.05）.It is concluded that BM-MSC from MM patients express HGF,SDF-1,MCP-1,IL-8,but these chemotaxis-related factors expression of bone marrow microenvironment cellular component are dysregulated in MM patients,which may result from the interplay between MM cells and MSC.

关 键 词：多发性骨髓瘤 骨髓间充质干细胞 趋化相关因子 

分 类 号：R733.3[医药卫生—肿瘤]