磷酸二酯酶7B在套细胞淋巴瘤患者中的表达及临床意义  

Expression of Cyclic Nucleotide Phosphodiesterase 7B in Patients with Mantle Cell Lymphoma and Its Prognostic Significance

作　　者：董华洁[1] 范磊[1] 王冬梅[1] 方成[1] 朱丹霞[1] 王银花[1] 洪鸣[1] 朱雨[1] 乔纯[1] 庄芸[1] 徐卫[1] 李建勇[1] 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院血液科,江苏南京210029

出　　处：《中国实验血液学杂志》2011年第1期94-99,共6页Journal of Experimental Hematology

基　　金：国家自然基金资助项目(编号30871104,30971296,30971295);江苏省医学领军人才基金资助项目(编号LJ200623);江苏省医学重点人才基金资助项目(编号RC2007042);江苏省“青蓝工程”中青年学术带头人培养对象项目;江苏省“六大人才高峰”资助项目

摘　　要：为了研究磷酸二酯酶(PDE)7B在套细胞淋巴瘤(MCL)患者中的表达及临床意义,应用实时定量PCR(QPCR)技术检测20例初治的有骨髓侵犯MCL患者骨髓和20例正常人外周血单个核细胞中pde7b的表达水平,并分析其与MCL临床预后指标的关系。结果表明:20例MCL患者pde7b表达水平中位数为8.7×10-4(4×10-5-6.9×10-3),明显高于正常人群0.5×10-4(0.18×10-4-1.7×10-4)(p=0.001);pde7b与性别、年龄、白细胞计数、乳酸脱氢酶水平、CD38表达和免疫球蛋白重链基因突变均无明显相关,而与细胞遗传学、β2微球蛋白、ZAP-70表达有明显相关。结论:pde7b基因在MCL中表达明显高于正常人群,并与细胞遗传学关系密切,可能成为MCL新的预后指标,具有重要的临床意义。To investigate the expression level of cyclic nucleotide phosphodiesterase（PDE） 7B mRNA and its prognostic value in mantle cell lymphoma（MCL）,the real-time quantitative RT-PCR（QPCR） was used to detect pde7b expression levels of bone marrow mononuclear cells from 20 newly diagnossed MCL patients with bone marrow involvement and peripheral blood mononuclear cells from 20 normal persons,the association of pde7b expression levels with prognostic indexes was analyzed by statistical software.The results showed that the median values of pde7b mRNA expression level in 20 MCL patients and normal controls were 8.7×10-4（4×10-5-6.9×10-3） and 0.5×10-4（0.18×10-4-1.7×10-4） respectively（p=0.001）.No association was found between pde7b expression and patients′ clinical baseline information（gender and age）,as well as certain prognostic factors,leukocyte count,lactate dehydrogenase level,CD38 expression and immunoglobulin heavy-chain variable region mutation status,but pde7b mRNA expression was significantly associated with cytogenetic abnormality,β2-microglobulin level and ZAP-70 expression.It is concluded that the pde7b mRNA expression is obviously higher in MCL patients compared with normal controls and significantly correlates with unfavorable cytogenetic characteristics in MCL.The pde7b may be used as a novel prognostic indicator in MCL,and has important clinical significance.

关 键 词：套细胞淋巴瘤 磷酸二酯酶7B 实时定量PCR 

分 类 号：R733.1[医药卫生—肿瘤]