PHI对Burkitt淋巴瘤Daudi细胞株组蛋白甲基化和乙酰化调控的实验研究  

PHI Regulates Histone Methylation and Acetylation in Burkitt Lymphoma Daudi Cell Line

作　　者：洪苓苓[1] 马旭东[2] 黄轶群[2] 

机构地区：[1]福建省漳州市中医院内科,福建漳州363000 [2]福建医科大学附属漳州市医院血液科,福建漳州363000

出　　处：《中国实验血液学杂志》2011年第1期105-108,共4页Journal of Experimental Hematology

基　　金：卫生部研究基金福建卫生教育联合攻关计划项目(编号wkj2008-2-55);福建医科大学科学研究发展专项基金计划资助项目(编号FZS08018);漳州市科学研究发展计划基金资助项目(编号Z07014)

摘　　要：本研究观察异硫氰酸苯己酯(PHI)在体外对Burkitt淋巴瘤Daudi细胞株的作用及对淋巴瘤细胞表观遗传学调控的影响,初步探讨其可能的机制。用流式细胞术检测PHI处理后的细胞凋亡率,蛋白免疫印迹法检测PHI处理后的细胞的组蛋白H3、H4乙酰化和H3K4、H3K9甲基化状态改变。结果表明,PHI诱导细胞凋亡并提高组蛋白H3、H4乙酰化水平,组蛋白H3K4甲基化增强,而H3K9甲基化减弱。结论:PHI能上调与转录激活相关的组蛋白H3乙酰化水平及甲基化H3K4,下调转录抑制相关的组蛋白甲基化H3K9,促进肿瘤细胞凋亡,PHI可作为淋巴瘤的靶向治疗药物。This study was purposed to investigate the effects of phenylhexyl isothiocyanate（PHI） on Burkitt lymphoma Daudi cell line and regulation of histone acetylation and methylation in Daudi cells,and to explore the potential mechanism.The apoptotic rate of Daudi cells treated with PHI was measured by flow cytometry,the changes of histone H3 and H4 acetylation,histone H3K9 and H3K4 methylation in Daudi cells treated with PHI were detected by Western blot.The results showed that PHI could induce apoptosis of Daudi cells,increased the acetylation level of H3 and H4,enhanced the methylation of H3K4,but reduced the methylation of H3K9.It is concluded that the PHI can up-regulate the acetylation level of histone H3 associated with transcription stimulation and the methylation of histone H3K4,down-regulate the methylation on histone H3K9 associated with transcription inhibition,promotes the apoptosis of Daudi cells.PHI may be a potential agent for target therapy of lymphoma.

关 键 词：异硫氰酸苯己酯 BURKITT淋巴瘤 Daudi细胞株 组蛋白甲基化 组蛋白乙酰化 

分 类 号：R733.1[医药卫生—肿瘤] R979.1[医药卫生—临床医学]