星形细胞瘤中血管生成拟态的鉴定及其临床意义  

Identification of vasculogenic mimicry in astrocytoma and its clinical significance

作　　者：张熙[1] 郭世文[1] 尉春艳[2] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061 [2]西安交通大学医学院第二附属医院妇产科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2011年第2期205-207,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30672126)~~

摘　　要：目的明确星形细胞瘤中是否存在血管生成拟态及其临床意义。方法应用三维培养技术、HE染色、免疫组织化学与组织化学双重染色方法,观察星形细胞瘤中拟态血管的存在情况。结果星形细胞瘤细胞株三维培养后发生细胞重塑,彼此融合,相互连接形成网状结构;免疫组织化学与组织化学双重染色可见,Ⅰ、Ⅱ级星形细胞瘤中均未发现血管生成拟态;Ⅲ级星形细胞瘤中发现有3例存在血管生成拟态(3/18),血管生成拟态密度为(5.55±3.13)个/HP;Ⅳ级星形细胞瘤中发现有7例存在血管生成拟态(7/25),血管生成拟态密度为(8.81±2.84)个/HP;血管生成拟态随着恶性程度的增加明显增加,各组间差异具有统计学意义(P<0.05)。结论星形细胞瘤中存在血管生成拟态现象,血管生成拟态与肿瘤的恶性程度呈正相关。Objective To investigate vasculogenic mimicry（VM） in astrocytoma and its clinical significance.Methods Three-dimensional culture system of U87 cells was constructed to observe VM.The presence of VM in 65 specimens of astrocytoma was observed by immunohistochemical and histological double staining of CD34 and PAS.Results After three-dimensional culture,U87 cells remolded and fused with each other,forming wreath and network structure.The immunohistochemical and histological double staining showed that VM was present in astrocytoma.VM was not found in grade I and Ⅱ astrocytoma.The positive rate of VM in grade Ⅳ group（7/25） was significantly higher than that in grade Ⅲ group（3/18）.VM density was 8.81±2.84/HP in grade Ⅳ group and 5.55±3.13/HP in grade Ⅲ group;it increased with the increase of malignancy.There were significant differences between the groups.Conclusion VM is present in astrocytoma and it is correlated with the malignancy.

关 键 词：星形细胞瘤 血管生成拟态 三维培养 CD34-PAS双重染色 恶性程度 

分 类 号：R739.41[医药卫生—肿瘤]