兔眼葡萄膜巩膜引流术后FITC-Dextran在眼组织中的分布  

Distribution of FITC-dextran in rabbit eye tissues after uveoscleral drainage surgery

作　　者：雷剑琴[1] 孙乃学[2] 樊小娟[1] 陈丽[1] 

机构地区：[1]西安交通大学医学院第一附属医院眼科,陕西西安710061 [2]西安交通大学医学院第二附属医院眼科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2011年第2期242-245,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的观察兔眼葡萄膜巩膜引流术后房水流出道的形态学改变。方法 20只中国白兔随机分为2组,一组行葡萄膜巩膜引流术,另一组行小梁切除术,均行右眼手术。术后2周术眼前房内灌注异硫氰酸荧光素标记的葡聚糖(FITC-Dextran),处死后取完整眼球做冰冻切片,在激光共聚焦显微镜下观察荧光素的分布。与手术部位处在眼环同一子午线上的同眼的对称部位未手术眼组织作为对照。结果在葡萄膜巩膜引流术组,荧光素的分布在前、中部巩膜最强,前葡萄膜次之;而在小梁切除术组,荧光素的分布在前葡萄膜最强,前、中部巩膜次之,两组的后葡萄膜和后巩膜均有部分中等亮度的显影,对照组的后葡萄膜和后巩膜则几乎无显影,且两组的前、中部巩膜荧光素强度均明显高于对照组。结论葡萄膜巩膜引流术和小梁切除术术后房水引流均是多途径的,既有外滤过,还增强了葡萄膜巩膜引流。与小梁切除术相比,葡萄膜巩膜引流术还明显增加了跨巩膜引流。Objective To study the morphologic changes of aqueous humor outflow pathway after uveoscleral drainage surgery.Methods Totally 20 Chinese white rabbits were randomly divided into two groups.Uveoscleral drainage surgery was performed in one group and trabeculectomy was performed in the other;only the right eyes were chosen in both groups.Two weeks after surgery,FITC-dextran was perfused intracamerally on the operated eyes.After the rabbits were sacrificed the whole eyeball was extracted and prepared for frozen sections.Distribution of fluorescein was observed under confocal microscope.The normal tissue opposite to the surgical site which was untouched was used as control.Results In uveoscleral drainage group,fluorescein was most expressed in the anterior and middle parts of the sclera,followed by the anterior uveal tract.In trabeculectomy group,however,fluorescein was most expressed in the anterior uveal tract,and anterior and middle parts of the sclera followed as the second.In both groups,only parts of the posterior sclera and uveal tract were moderately manifested,but hardly any fluorescein could be seen in these parts in their corresponding controls.Fluorescein in the anterior and middle parts of sclera was significantly brighter than that in controls in both groups.Conclusion There seems to be a multi-pathway of aqueous humor drainage after both trabeculectomy and our devised surgery.There is not only a flow out but also an enhanced uveoscleral outflow.Compared with trabeculectomy,uveoscleral drainage surgery may also increase transcleral outflow.

关 键 词：葡萄膜巩膜引流术 小梁切除术 异硫氰酸荧光素标记的葡聚糖(FITC-Dextran) 兔 激光共聚焦显微镜 

分 类 号：R775[医药卫生—眼科]