Bmi-1基因过度表达与喉癌的分化、转移及预后的关系  

Association of Bmi-1mRNA overexpression with differentiation,metastasis and prognosis of laryngocarcinoma

作　　者：姚小宝[1] 王晓侠[2] 张少强[1] 闫利英[1] 朱宏亮[1] 

机构地区：[1]西安交通大学医学院第一附属医院耳鼻喉科,陕西西安710061 [2]解放军451医院耳鼻喉科,陕西西安710054

出　　处：《西安交通大学学报（医学版）》2011年第2期246-249,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金国际合作项目资助(No.30210103151)~~

摘　　要：目的探讨喉癌组织中Bmi-1基因mRNA的表达与喉癌的分化、转移及临床预后的关系。方法收集我们医院2008年5月~2008年11月共52例行喉癌手术切除的标本,提取肿瘤组织及癌旁组织配对标本的总RNA,采用RT-PCR方法测定Bmi-1基因mRNA的表达,Western blot法分析Bmi-1蛋白的表达水平,并结合临床资料对Bmi-1基因差异表达与喉癌患者的临床表现进行相关性分析。结果 52例配对标本分别进行Bmi-1mRNA荧光检测比较,39例标本的肿瘤组织中Bmi-1基因mRNA的表达明显高于癌旁正常喉组织;32例标本的肿瘤组织中Bmi-1蛋白表达明显高于癌旁正常喉组织;Bmi-1mRNA的表达与喉癌淋巴结转移和浸润深度密切相关（P〈0.05）,而与患者的性别、年龄、吸烟及肿瘤分化程度等无关（P〉0.05）。结论 Bmi-1基因在喉癌组织中的表达状态与喉癌的生长和浸润转移关系密切;Bmi-1基因mRNA可望作为喉癌病情发展及指导临床治疗的标记物之一;Bmi-1mRNA的测定有助于判断肿瘤预后。Objective To investigate the association of B cell-specific MLV integrationsite-1（Bmi-1） mRNA expression level with the differentiation,metastasis and prognosis of laryngocarcinoma.Methods Tissue specimens were obtained from 52 patients undergoing surgery for laryngocarcinoma between May and November 2008 in our hospital.Reverse transcriptional PCR（RT-PCR） was performed for amplification of Bmi-1 mRNA from the 52 tumor tissues and matched adjacent normal tissues,and the differential Bmi-1 mRNA expression was analyzed for its association with the clinical manifestations of the patients.Results Bmi-1 mRNA and protein expressions were detected in all the laryngocarcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method,and in 39 cases Bmi-1 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues.In 32 cases of tumor tissue,Bmi-1 protein expression was significantly higher than the adjacent normal laryngeal tissue.The expression of Bmi-1 mRNA was highly correlated with tumor size,lymph node metastasis and T classification（P0.05）,but not with the patients＇ gender,age or tumor differentiation（P0.05）.Conclusion Bmi-1 mRNA expression is closely correlated with the differentiation and metastasis of laryngocarcinoma.Bmi-1 mRNA may serve as a marker for assessing the progression of laryngocarcinoma and providing assistance for clinical therapeutic decisions and may facilitate its prognostic evaluation.

关 键 词：喉癌 BMI-1 基因表达 肿瘤转移 预后 

分 类 号：R736.3[医药卫生—肿瘤]