葛根素对肝缺血再灌注损伤所致心功能障碍的保护作用及机制  

Protective effect of puerarin on cardiac insufficiency during liver is chemia-reperfusion injury in rats

作　　者：邵亚芳[1] 徐群[1] 沈艳艳[1] 王旭林[2] 王红梅[3] 赵传昌[4] 吴仲敏[5] 

机构地区：[1]台州学院医学院,浙江台州318000 [2]台州市立医院肿瘤外科,浙江台州318000 [3]台州学院医学院病理生理学教研室,浙江台州318000 [4]台州学院医学院实验中心,浙江台州318000 [5]台州学院医学院解剖学教研室,浙江台州318000

出　　处：《中国现代普通外科进展》2011年第1期20-23,共4页Chinese Journal of Current Advances in General Surgery

基　　金：浙江省2008大学生"新苗人才计划"基金(2008R40G2170048)

摘　　要：目的:观察葛根素预处理在肝缺血再灌注损伤中对心功能的保护作用及机制。方法:建立肝缺血再灌注损伤动物模型,选健康雄性SD大鼠32只,随机分为对照组、缺血再灌注组(I/R组)、葛根素预处理组(PUE组)和生理盐水预处理组(N组),每组8只。测定心功能指标(HR、LVSP、LVEDP、+dp/dtmax、-dp/dtmax、血清内毒素、MPO)、血清内毒素含量及心肌MPO活力。结果:HR在I/R组与对照组相比差异无统计学意义(P>0.05),PUE组和N组HR较I/R组有所增加,但其差异无统计学意义(P>0.05),N组和PUE组相比差异亦无统计学意义(P>0.05)。I/R组LVSP、+dp/dtmax较对照组明显下降(P<0.05),PUE组较对照组和I/R组明显升高(P<0.01)。LVEDP、-dp/dtmax在PUE组明显低于对照组和I/R组(P<0.01)。血清内毒素的含量及心肌MPO活力I/R组与对照组比较明显增加,差异有统计学意义(P<0.01),PUE组与对照组、I/R组比较明显降低,差异有统计学意义(P<0.01),N组与PUE组比较差异无统计学意义(P>0.05)。结论:肝缺血再灌注损伤可引起心功能障碍,葛根素通过降低血清内毒素含量及心肌MPO活力可改善心功能指标。Objective： To explore protective effect of puerarin on cardiac insufficiency following liver ischemia-reperfusion injury.Methods： A model of liver ischemia-reperfusion injury was established by imitation.Thirty-two healthy rats were randomly divided as following（n=8）： the control group（the group of sham operation）,reperfusion following ischemia 30 min（I/R group）,puerafin pretreatment group（PUE group）,normal saline pretreatment group（N group）.HR,LVSP,LVEDP,＋dp/dtmax,-dp/dtmax,endotoxin and MPO were observed.Results： HR was no significant statistics between I/R group and N group（P0.05）,and increased in PUE group and N group,compared with I/R group.The contents of LVSP,LVEDP and ＋dp/dtmax were significantly decreased between I/R group and N group（P0.05）,remarkable increasing in PUE group（P0.01）,the contents of LVEDP,-dp/dtmax were significantly decreased in PUE group,compared with I/R and N group.Endotoxin levels and MPO were significantly increased between I/R group and N group（P0.05）,remarkable decreasing in PUE group（P0.05）,but no significant statistics between PUE group and N group（P0.05）.Conclusion： Cardiac insufficiency may be induced following the process of liver ischemia-reperfusion injury,then puerafin pretreatment have an protect effect on cardiac insufficiency by decreasing endotoxin levels and MPO.

关 键 词：肝缺血再灌注损伤 葛根素 心功能障碍 内毒素 髓过氧化物酶 

分 类 号：R657.3[医药卫生—外科学]