高压氧对结肠癌细胞缺氧诱导因子-1α和血管内皮生长因子mRNA表达的影响  

Effects of hyperbaric oxygen on the expression of hypoxia inducible factor-1α mRNA and vascular endothelial growth factor mRNA in colon adenocarcinoma cells

作　　者：张丽达[1] 马里[1] 田小强[2] 张伟[2] 黄平[1] 钱霞[1] 杨琳[2] 

机构地区：[1]东南大学附属中大医院,南京210009 [2]东南大学医学院,南京210009

出　　处：《中华物理医学与康复杂志》2011年第2期87-90,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：江苏省教育厅科研基金项目（H.103-059）、南京市社会发展科技项目（200401070-3,20080439）资助

摘　　要：目的初步探讨高压氧（HBO）对结肠癌细胞缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）mRNA表达的影响。方法将结肠癌细胞SW480分为正常对照组、高压氧不同压力（0．15MPa、0．20MPa、0．25MPa）组和高压氧不同暴露时间（12h、24h、36h）组，各组分别处理后，采用逆转录聚合酶链反应（RT—PCR）检测不同压力及时间HBO暴露后结肠癌SW480细胞的HIF-1α和VEGF mRNA表达水平改变。结果SW480细胞在0．25 MPa HBO暴露后12hHIF-1α mRNA表达水平较0．15MPa和0．20MPa HBO暴露后12h为低（P〈0．01），0．25MPaHBO暴露后24h HIF-1α mRNA表达缺失；0．25 MPa HBO暴露后36h VEGF mRNA表达较0．15MPa、0．20MPa HBO暴露后12h和24h为低（P〈0．05）。结论HBO可能通过下调HIF-1α mRNA、VEGF mRNA来抑制肿瘤组织的血管形成。Objective To investigate the effects of hyperbaric oxygen （HBO） on the expression of hypoxia inducible factor-1α （HIF-1α） mRNA and vascular endothelial growth factor （VEGF） mRNA in colon adenoeareinoma cells, Methods 5W480 colon cancer cells were divided into a control group and an HBO group, which was in turn divided into various subgroups according to the various HBO pressures （0.15 MPa, 0.20 MPa, 0.25 MPa） and exposure times （ 12, 24 and 36 h） tested. The expression of HIF-1α and VEGF mRNA was evaluated with RT-PCR after /he various treatments. Results The expression of HIF-1α mRNA was lower in the SW480 cells than in the controls after exposure to 0. 25 MPa HBO for 12 h. No expression of HIF-1α mRNA was detected after exposure to 0.25 MPa HBO for 24 h. The expression of VEGF mRNA was lower after exposure to 0.25 MPa HBO for 36 h than in the controls. Conclusion Absence of HIF-1α mRNA expression and decreased expression of VEGF mRNA in 5W480 can be observed after exposure to 0.25 MPa HBO for 24 h, suggesting that HBO may inhibit the formation of tumor blood vessels through down-regulating the expression of these mRNAs.

关 键 词：高压氧 结肠癌细胞 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R735.35[医药卫生—肿瘤]