芝麻素对代谢综合症大鼠心肌NT、NF-κB和MMP-9蛋白表达的影响  

Sesamin represses protein expression of nitrotyrosine,NF-κB and matrix metalloproteinase-9 in myocardium of metabolic syndrome rats

作　　者：孔祥[1] 杨解人[1] 张明义[1] 吴向起[1] 

机构地区：[1]皖南医学院药理学教研室,国家中医药管理局中药药理三级实验室,安徽芜湖241002

出　　处：《中国药理学通报》2011年第3期373-377,共5页Chinese Pharmacological Bulletin

基　　金：安徽省教育厅自然科学研究基金重点项目(No2006KJ099A);芜湖市2008年度科技计划重点项目(No2008512)

摘　　要：目的探讨芝麻素改善代谢综合症(metabolic syndrome,MS)大鼠心肌重构的作用机制。方法采用两肾一夹术伴高脂高糖饮食制备MS大鼠模型,灌服不同剂量芝麻素(120、60、30 mg.kg-1.d-1)8周后处死动物,称全心湿重和左心室湿重,测血清总抗氧化能力(total antioxidant capability,T-AOC),Western blot检测心肌硝基酪氨酸(nitroty-rosine,NT)、核因子-κB(NF-κB)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)蛋白表达,HE和Masson染色观察心肌病理变化并测定胶原容积分数。结果与模型组相比,60、120 mg.kg-1芝麻素组全心湿重降低,T-AOC升高,心肌NT、NF-κB和MMP-9蛋白表达降低。120 mg.kg-1芝麻素组左心室湿重和胶原容积分数降低,心肌病理损伤明显改善。结论芝麻素具有改善MS大鼠心肌重构的作用,其机制除抗氧化应激外,还与下调NF-κB和MMP-9蛋白表达有关。Aim To investigate the possible in vivo protective effects of sesamin on myocardial remodeling in metabolic syndrome（MS） rats.Methods MS rat model was induced by operation of two-kidneys with one-clip and fed with a high-fat,high-sucrose diet.sesamin（120,60 and 30 mg·kg^-1·d^-1） was orally administered in MS rats.Eight weeks later,rats were sacrificed,heart wet weight（HWW） and left ventricle wet weight（LVWW） were recorded,total-antioxidative activity（T-AOC） in serum was measured,and protein expression of nitrotyrosine（NT）,NF-κB and matrix metalloproteinase-9（MMP-9） in myocardium was detected by Western blot.In addition,pathological changes in myocardium were observed with HE and Masson staining and collagen volume fraction（CVF） was measured.Results Compared with MS rats,treatment with 120 and 60 mg·kg-1 sesamin decreased HWW,enhanced T-AOC,and reduced NT,NF-κB and MMP-9 protein expression.Treatment with 120 mg·kg-1 sesamin decreased LVWW and CVF,and obviously ameliorated myocardial pathologic lesions.Conclusion Sesamin improves myocardial remodeling through reduction of NF-κB and MMP-9 expression in addition to alleviation of oxidative stress in MS rats.

关 键 词：芝麻素 代谢综合症 硝基酪氨酸 NF-ΚB 基质金属蛋白酶-9 心肌肥厚 

分 类 号：R-332[医药卫生] R284.1