CNTN-1、VEGF-C和VEGFR-3在胃癌组织中的表达及其临床意义  

Expressions and Significance of CNTN-1,VEGF-C,and VEGFR-3 in Gastric Cancer

作　　者：吴生华[1] 俞继卫[1] 郑林海[1] 吴巨钢[1] 倪晓春[1] 李晓强[2] 杜光烨[2] 姜波健[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院普外科,上海201900 [2]上海交通大学医学院附属第三人民医院病理科,上海201900

出　　处：《中国普外基础与临床杂志》2011年第2期142-148,共7页Chinese Journal of Bases and Clinics In General Surgery

基　　金：上海市科委基金(项目编号:09411962300);上海交通大学医学院科研基金(项目编号:2007XJ032;2009XJ21037)~~

摘　　要：目的探讨神经细胞黏附分子———接触蛋白-1(CNTN-1)、血管内皮生长因子-C(VEGF-C)及其受体VEGFR-3(Flt-4)在原发性胃癌组织中的表达,并分析三者的相关性及其与临床病理特征的关系。方法选取68例原发性胃癌患者肿瘤原发灶和正常胃黏膜组织标本,行免疫组化染色检测VEGF-C、VEGFR-3和CNTN-1蛋白表达,测定VEGFR-3阳性淋巴管密度(FVD)和D2-40阳性淋巴管密度(LVD)。结果胃癌组织中VEGF-C、VEGFR-3和CNTN-1蛋白表达阳性率分别为57.4%(39/68)、60.3%(41/68)和55.9%(38/68),显著高于正常胃黏膜组织〔20.6%(14/68)、23.5%(16/68)、16.2%(11/68)〕,P=0.000。在TNM分期较晚、发生淋巴管浸润和淋巴结转移的患者其肿瘤组织中三者的表达阳性率均较高(P<0.05)。CNTN-1蛋白表达与VEGF-C(r=0.372,P=0.002)及VEGFR-3蛋白(r=0.308,P=0.011)表达之间呈正相关。68例患者肿瘤组织中FVD为(10.41±9.38)个/HP,明显低于其LVD〔(18.19±7.44)个/HP〕,P=0.000。在TNM分期较晚、有淋巴管浸润和淋巴结转移的患者肿瘤组织中,FVD与LVD均较高(P<0.05)。VEGF-C和CNTN-1蛋白表达阳性者FVD明显高于表达阴性者(P=0.029和P=0.003);LVD与CNTN-1(P=0.727)、VEGF-C(P=0.173)和VEGFR-3蛋白(P=0.924)表达均无关。VEGF-C、VEGFR-3及CNTN-1蛋白表达阳性患者术后生存率分别较表达阴性患者低(P<0.05)。结论 CNTN-1蛋白在胃癌组织中高表达,与VEGF-C和VEGFR-3蛋白表达相关,联合检测对判断胃癌侵袭性和预后有重要参考价值。VEGF-C可能通过CNTN-1通路介导淋巴管生成,从而促进胃癌的淋巴浸润。Objective To investigate the expressions of contactin-1（CNTN-1）,vascular endothelial growth factor-C（VEGF-C）,and its receptor VEGFR-3（Flt-4） in primary gastric cancer and to explore the relevance among them and their correlation with clinicopathologic features of gastric cancer.Methods The VEGF-C,VEGFR-3,and CNTN-1 protein expressions of tumor tissues and normal gastric mucosa tissues in 68 patients with primary gastric cancer were analyzed by immunohistochemistry.The Flt-4-positive vessel density（FVD） and lymphatic vessel density（LVD） were also analyzed by VEGFR-3-positive and D2-40-positive staining,respectively.Results The positivity rate of VEGF-C,VEGFR-3,and CNTN-1 protein expression in the primary tumor was 57.4%（39/68）,60.3%（41/68）,and 55.9%（38/68）,respectively,which was significantly higher than that in the normal gastric mucosa tissues（20.6%（14/68）,23.5%（16/68）,and 16.2%（11/68））,P=0.000.The expressions of VEGF-C,VEGFR-3,and CNTN-1 protein were significantly correlated with TNM stage,lymphatic vessel invasion,and lymph node metastasis（P0.05）.The expression of CNTN-1 protein was significantly correlated with VEGF-C（r=0.372,P=0.002） and VEGFR-3 protein expression（r=0.308,P=0.011）.In tumor tissues of sixty-eight patients the FVD was（10.41±9.38）/HP,which was significantly lower than LVD（（18.19±7.44）/HP）,P=0.000.Elevated FVD and LVD was significantly found in patients with tumor characterized by later TNM stage,severer lymphatic vessel invasion,and severer lymph node metastasis（P0.05）.The FVD of tumor was significantly correlated with VEGF-C（P=0.029） and CNTN-1 protein expression（P=0.003）.The LVD of tumor was not significantly correlated with CNTN-1（P=0.727）,VEGF-C（P=0.173）,and VEGFR-3 protein expression（P=0.924）.The patients with positive expression of VEGF-C,VEGFR-3,and CNTN-1 protein showed poorer prognosis（P0.05）.Conclusions Elevated expression of CNTN-1 protein is observed in primary gastric cancer and co

关 键 词：胃癌 接触蛋白-1 血管内皮生长因子 血管内皮生长因子受体 D2-40 淋巴结转移 

分 类 号：R735.2[医药卫生—肿瘤]