角质细胞生长因子和环氧化酶-2在胃癌中的表达及其与血管生成的关系  

Expression of Keratinocyte Growth Factor and Cyclooxygenase-2 in Gastric Cancer and Its Correlation with Angiogenesis

作　　者：陈振栋[1] 谭浪平[1] 王亚洲[1] 孙岩松[1] 邱堃[2] 

机构地区：[1]佳木斯大学第一附属医院普外一科,黑龙江佳木斯154002 [2]海南省第二人民医院外二科,海南五指山572200

出　　处：《中国普外基础与临床杂志》2011年第2期177-182,共6页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的检测角质细胞生长因子(KGF)和环氧化酶-2(COX-2)蛋白在胃癌组织中的表达及微血管密度(MVD),探讨KGF与COX-2在胃癌发生、发展中的作用及其机理。方法采用免疫组化SP法检测64例胃癌组织及30例正常胃黏膜组织中KGF和COX-2蛋白表达,并采用CD34抗体染色检测MVD。结果 KGF和COX-2蛋白在胃癌组织中的表达阳性率分别为65.6%(42/64)和79.7%(51/64),分别高于其在正常胃黏膜组织中的表达阳性率〔23.3%(7/30)和13.3%(4/30)〕,P=0.046、P=0.008。胃癌组织MVD为31.8±8.0,明显高于正常胃黏膜组织的14.3±6.1(P=0.000);KGF与COX-2蛋白均表达阳性者MVD为35.9±5.7,明显高于两者表达均为阴性者的25.7±7.0(P=0.000)。胃癌组织中KGF和COX-2蛋白表达均与淋巴结转移、浆膜浸润及TNM分期有关(P<0.05、P<0.01),MVD与淋巴结转移和TNM分期有关(P<0.01),但均与患者年龄、性别以及肿瘤分化程度无关(P>0.05)。KGF与COX-2联合表达者与胃癌的浆膜浸润、淋巴结转移和TNM分期有关(P<0.05),与患者年龄、性别以及肿瘤分化程度无关(P>0.05)。胃癌组织中KGF与COX-2蛋白表达呈正相关(r=0.610,P=0.000);胃癌组织MVD与KGF和COX-2蛋白表达均呈正相关(r=0.675,P=0.000;r=0.657,P=0.000)。结论 KGF和COX-2蛋白在胃癌组织中高表达,可能通过促进肿瘤新生血管的生成协同参与胃癌的浸润、转移。Objective To investigate the expression of keratinocyte growth factor（KGF） and cyclooxygen-ase-2（COX-2） protein and microvessel density（MVD）,and to explore their function and mechanism in the multi-step process of gastric cancer.Methods The expressions of KGF and COX-2 protein in 64 samples of gastric cancer and 30 cases of normal gastric mucosa tissues were detected by immunohistochemistry.The MVD was detected by staining the endothelial cells in microvessles using anti-CD34 antibody.Results The positive rate of KGF and COX-2 protein expression in gastric cancer were 65.6%（42/64） and 79.7%（51/64）,respectively,which was significantly higher than that in normal gastric mucosa tissues（（23.3%,7/30）,P=0.046;（13.3%,4/30）,P=0.008）.The MVD of gastric cancer was 31.8±8.0,which was significantly higher than that of normal gastric mucosa tissues（14.3±6.1）,P=0.000.The MVD in gastric cancer with coexpressive KGF and COX-2 protein was 35.9±5.7,which was significant higher than that with non-coexpressive KGF and COX-2 protein（25.7±7.0）,P=0.000.Both the expression of KGF and COX-2 protein were related to the invasion of serosa,lymph node metastasis and TNM staging（P0.05,P0.01）.The MVD of gastric cancer tissues was related to lymph node metastasis and TNM staging（P0.05）,but unrelated to patient＇s age,gender,and differentiation of tumor （P0.05）.The co-expression of KGF and COX-2 protein was frequently found in patients with deeper invasion of serosa,lymph node metastasis,and higher TNM staging（P0.05）,but which was not associated withpatient＇sage,gender,and differentiation of tumor（P0.05）.The expression of KGF protein was positively correlated to the expression of COX-2 protein（r=0.610,P=0.000）.There was positive correlation between MVD and the expression of KGF（r=0.675,P=0.000） and COX-2 protein（r=0.657,P=0.000） in gastric cancer,respectively.Conclusion KGF and COX-2 highly expressed by gastric cancer,which may be involved in the invasion and metastas

关 键 词：胃癌 角质细胞生长因子 环氧化酶-2 微血管密度 血管生成 

分 类 号：R735.2[医药卫生—肿瘤]