热休克蛋白gp96-肽复合物体外对人肝癌细胞的抗肿瘤效应  

The anti-hepatoma effects of HSP gp96-peptide complexes in vitro

作　　者：吴信华[1] 庄红芹[2] 鞠少卿[1] 戚菁[1] 施维[1] 姚登福[3] 

机构地区：[1]南通大学附属医院外科综合实验室,226001 [2]南京大学医药生物技术国家重点实验室 [3]南通大学临床医学研究中心

出　　处：《江苏医药》2011年第4期409-411,共3页Jiangsu Medical Journal

基　　金：江苏省卫生厅重点项目(H200848);南通市社会发展科技计划项目(S2008053)

摘　　要：目的研究热休克蛋白gp96-肽复合物修饰树突状细胞(DC)后对人肝癌细胞的体外特异抗肿瘤效应。方法从人原发性肝癌组织中提取gp96-肽复合物,用其修饰DC细胞后与T淋巴细胞共同培养,以MTT法检测该复合物介导的细胞毒性T细胞(CTL)对不同来源人肝癌细胞的抗肿瘤效应,并与粗提抗原修饰DC细胞组相比较。结果 gp96-肽复合物诱导的CTL对原代肝癌细胞杀伤效率为74.3%,明显高于粗提抗原组(42.5%)和未加抗原组(14.4%)(P<0.01),粗提抗原组对肝癌细胞杀伤效率高于未加抗原组(P<0.01)。且该复合物的抗肿瘤效应具有一定的组织特异性。结论 gp96-肽复合物修饰的DC细胞,在体外能刺激产生特异的抗肿瘤细胞毒性T细胞,因而热休克蛋白gp96在肿瘤免疫治疗中具有重要的实用价值。Objective To investigate the specific anti-hepatoma effects of dendritic cells（DCs） modified by HSP gp96-pepetide complexes in vitro.Methods Gp96-peptide complexes were obtained from hepatocellular carcinoma （HCC） tissues.DCs were modified by the complexes and co-cultured with T lymphocytes.The effects of anti-hepatoma by the complexes mediated CTL were detected by MTT method,and then compared with the effects induced by DCs modified crudely extracted antigen.Results The killing effectiveness rate of primary HCC cells induced by CTL in gp96-peptide complexes group was 74.3%,which was significantly higher than 42.5% in crudely extracted antigen group and 14.4% in no antigen group（P0.01）.The killing effectiveness rate of crudely extracted antigen group was higher than that in no antigen group（P0.01）.The killing effects had a tissue specificity.Conclusion DCs modified by gp96-peptide complexes could induce the specific anti-tumor effects mediated by CTL in vitro.HSP gp96 would play an important role in tumor immunotherapy.

关 键 词：GP96-肽复合物 树突状细胞 肝癌 

分 类 号：R735[医药卫生—肿瘤]