口腔扁平苔藓T细胞受体BV基因的优势取用初探  

T cell receptor BV gene preferential usage in oral lichen planus

作　　者：姚辉[1] 李慧源[2] 周永梅[1] 沈雪敏[1] 唐国瑶[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔黏膜科,上海市口腔医学重点实验室,上海200011 [2]中日友好医院口腔科,北京100029

出　　处：《上海口腔医学》2011年第1期66-69,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30872888);上海市科学技术委员会科研计划(06ZR14060);上海市重点学科建设项目(S30206)~~

摘　　要：目的:研究口腔扁平苔藓(oral lichen planus,OLP)患者损害组织中浸润的T细胞受体(T cell receptor,TCR)BV基因的取用格局。方法:采用实时定量聚合酶链反应,分析OLP患者损害组织中浸润的T细胞和外周血中T细胞TCR BV基因的取用格局。采用GraphPad Prism version 5.00软件对数据进行Mann-Whitney U检验。结果:43例OLP患者的损害组织对BV4、BV14和BV18基因的取用显著高于39例OLP患者外周血对这些BV基因的取用。结论:OLP患者损害组织浸润的T细胞优势取用BV4、BV14和BV18基因,提示带有BV4、BV14和BV18基因的T细胞参与了OLP的发病。PURPOSE： To identify the T cell receptor（TCR） structural characteristics of infiltrating T cells derived from the lesional tissues of oral lichen planus（OLP） patients.METHODS： BV gene distribution analysis was performed quantitatively in OLP patients using real-time polymerase chain reaction.The data was analyzed by Mann-Whitney U test using GraphPad Prism version 5.00 software.RESULTS： The usage toward BV4,BV14 and BV18 in the lesional tissues from 43 OLP patients was significantly higher than the usage toward these BV genes in the peripheral blood from 39 OLP patients.CONCLUSION： Infiltrating T cells derived from the lesional tissues in a cohort of Chinese OLP patients display preferential usage toward BV4,BV14 and BV18.It indicates that the T cells bearing BV4,BV14 and BV18 genes play a very important role in the etiology and pathogenesis of OLP.

关 键 词：口腔扁平苔藓 BV基因 T细胞 T细胞受体 

分 类 号：R781.5[医药卫生—口腔医学]