两株河口湿地耐盐石油降解菌的生物学特性及降解能力研究  

A STUDY ON THE BIOLOGICAL PROPERTIES AND DEGRADABILITY OF TWO SALT TOLERANT PETROLEUM-DEGRADING BACTERIA FROM ESTUARY WETLAND

作　　者：张竹圆[1,2] 白洁[1,2] 周方[1,2] 蔡忠亚[1] 赵阳国[1,2] 田伟君[1,2] 

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]海洋环境与生态教育部重点实验室,山东青岛266100

出　　处：《海洋湖沼通报》2011年第1期147-153,共7页Transactions of Oceanology and Limnology

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07208-009);国家重点基础研究发展规划(973)项目(2006CB400602)

摘　　要：从辽河口湿地污染土壤样品中分离筛选出2株高效石油降解菌lhk-2和lhk-10,经鉴定lhk-2为巴氏葡萄球菌（Staphylococcus pasteuri）,lhk-10为嗜麦芽寡养单胞菌（Stenotrophomonas maltophili）。两株菌的石油降解能力研究结果表明,培养8d后,加菌组的石油烃降解率均在60%以上。菌株lhk-2在12℃、pH5.0~9.0、NaCl浓度为2.5%的条件下生长良好;菌株lhk-10在12℃、pH5.0~10.0、NaCl浓度为3.0%时生长良好。培养20d后,两株菌对石油中所有正构烷烃组分去除率均在85%以上。Petroleum-degradating strains lhk-2 and lhk-10 obtained from the oil-contaminated siol samples from Liaohe Estuary,were identified as Staphylococcus pasteuri and Stenotrophomonas maltophili respectively.A study on their petroleum degradability finds that in the shake-flask experiment,the removal efficiencies of both starins were over 60 % after 8 days.In addition,the optimum culture condition was temperature of 12 C,pH of 5.0-9.0 and salitity of 25 for strain lhk-2 while temperature of 12oC,pH of 5.0-10.0 and salitity of 30 for strain lhk-10.And both lhk-2 and lhk-10 can achieve over 85% removal efficiency of n-alkanes after 20 days.

关 键 词：石油降解菌 河口湿地 石油污染 

分 类 号：X172[环境科学与工程—环境科学]