海参虫草复剂对免疫功能低下小鼠的免疫调节作用  

Immunomodulatory effects of a mixture of Apostichopus japonicus and Cordyceps militaris on immunocompromized mice

作　　者：王静凤[1] 李晓林[1] 滕来宾[1] 薛勇[1] 傅佳[1] 赵芹[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《卫生研究》2011年第2期215-218,共4页Journal of Hygiene Research

基　　金：海洋公益性行业科研专项(No.201105029);国家自然科学基金(No.30972284;30871944)

摘　　要：目的采用免疫功能低下小鼠模型探讨海参虫草复剂的免疫调节作用。方法 Balb/c小鼠随机分成正常对照组、模型对照组、阳性对照组和低、高剂量组,每组10只。剂量组小鼠灌胃不同浓度海参虫草复剂(60和120 mg/kg),阳性对照组灌胃左旋咪唑(20 mg/kg),正常和模型对照组灌胃生理盐水,连续28d。除正常对照组外,其余小鼠均于首次给药第21d,连续7d皮下注射氢化可的松(22.5 mg/kg),建立免疫功能低下小鼠模型。于末次给药后,分别测定小鼠的足趾增重量、抗体含量和抗体细胞生成数、碳廓清指数以及细胞因子γ-干扰素(IFN-γ)和白介素-4(IL-4)水平。结果海参虫草复剂能显著提高免疫低下模型小鼠的迟发性变态反应水平(P<0.01),提高血清溶血素含量(P<0.01)和抗体生成细胞数(P<0.05),促进巨噬细胞的吞噬能力(P<0.01);显著提高血清IFN-γ水平(P<0.01),降低IL-4水平(P<0.05,P<0.01),提高IFN-γ/IL-4比值(P<0.01)。结论海参虫草复剂对免疫功能低下模型小鼠的特异性和非特异性免疫机能具有显著调节作用。Objective To evaluate the immunomodulatory effect of a mixture of A.japonicus and C.militaris on immune functions of immunocompromized mouse model.Methods Fifty Balb/c mice were randomly selected and assigned into five groups（ten animals per group）： a normal control group,a model control group,a positive control group and low and high dose groups.A mixture of A.japonicus and Cordyceps militaris was administered orally every day to mice in dose groups at a dosage of 60 and 120 mg/kg.bw respectively.Mice in positive control group were given orally with levamisole hydrochloride at a dosage of 20mg/kg.bw.Mice in normal and model control groups were administered with 0.9% NaCl solution as placebo.All agents were administered once a day for 28 days.On the 21th day,all mice except that in the normal group were injected hypodermally with hydrocortisone at a dosage of 22.5mg/kg.bw once a day for 7 days to generate a immunocompromized animal model.The mice were deprived of food for 12h after the last administration,and the delayed type hypersensitivity（DTH）,hemolysin,antibody forming cells,the clearance of carbon particles in macrophages,the production of cytokine IFN-γ and IL-4 were measured respectively.Results The DTH level（P 0.01）,hemolysin content（P 0.01） and the number of antibody formaing cells（P 0.05）,carbon particle clearance index（P 0.01） in immunocompromized mice administered with the mixture of A.japonicus and C.militaris were increased obviously.At the same time,the mixture could also enhance IFN-γ（P 0.01） content and reduce IL-4（P 0.05） content.Conclusion Both the specific and non-specific immune function in immunocompromized mice could be stimulated by the mixture of A.japonicus and C.militaris.

关 键 词：海参 虫草 免疫功能 迟发性变态反应 血清溶血素 IFN-γ 

分 类 号：R965[医药卫生—药理学]