丹参山楂药对对大鼠动脉粥样硬化的影响  

Antiatherogenic effect of Radix Salviae Miltiorrhizae and Fructus Crataegi on experimental atherosclerosis in rats

作　　者：王伟[1] 杨滨[1] 王岚[1] 梁日欣[1] 陈承瑜[1] 胡楠[1] 成龙[1] 杨艳[1] 殷小杰[1] 高双荣[1] 叶锦霞 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]福建中医药大学中西医结合研究院,福建福州350108

出　　处：《中国中药杂志》2011年第6期784-789,共6页China Journal of Chinese Materia Medica

基　　金：科技部国际合作项目(2006DFB31720)

摘　　要：目的:观察丹参和山楂提取物(水浸膏)单煎及其合煎对大鼠动脉粥样硬化(AS)的影响。方法:Wistar大鼠随机分为对照组和造模组,造模组在高脂饮食的基础上采用腹腔注射VD3叠加卵清白蛋白激发免疫反应的方法诱发大鼠AS模型。造模8周确定造模成功后将造模组分为模型组、血脂康组、丹参组、山楂组、丹参山楂合煎剂组,各组灌胃给予相应药物4周。实验12周后检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平和超氧化物歧化酶(SOD)活力,丙二醛(MDA)含量,血管活性物质一氧化氮(NO)、血管内皮素(ET)、6-酮前列腺素F1α(6-keto-PGF1α)、血栓素B2(TXB2)以及炎性因子C反应蛋白(CRP)、白介素6(IL-6)、白介素8(IL-8)、肿瘤坏死因子α(TNF-α)含量。苏木素伊红(HE)染色观察主动脉组织形态学变化。结果:与模型组相比,各给药组TC,TG,LDL-C,MDA,ET,TXB2水平以及TXB2/PGF1α比值降低,HDL-C,SOD,NO,6-keto-PGF1α水平升高,CRP,IL-6,IL-8含量有不同程度的降低,对于TNF-α的释放未见明显影响。各药可见不同程度的抑制主动脉血管壁病理变化。结论:丹参山楂药对可以干预大鼠AS的形成,同时降低血脂水平,其作用可能与抗氧化作用及抑制炎性因子的释放有关。Objective： To investigate the antiatherogenic effect and possible mechanisms of the extracts of Radix Salviae Miltiorrhizae（RSM） or Fructus Crataegi（FC）,as well as their interaction.Method： Wistar rats were randomly divided into 2 groups： normal group and model group.The atherosclerotic model rats were injected VD3 and ovalbumin,while fed with high cholesterol diet.After the model was determined successfully,all model rats were divided into normal group,model group,Xuezhikang group,RSM group,FC group,mixture of RSM and FC group.Each group was given the corresponding drugs for 4 weeks.After 12 weeks,blood serum were analyzed for total cholesterol（TC）,triglyceride（TG）,low density lipop rotein cholesterol（LDL-C） and high density lipop rotein cholesterol（HDL-C）,superoxide dismutase（SOD）,malondialdehyde（MDA） and nitric oxide（NO）.And the blood plasma also analyzed for levels of endothelin（ET）,6-keto prostaglandin F1α（6-keto-PGF1α）,thromboxane B2（TXB2）,C-reactive protein（CRP）,interleukin 6（IL-6）,interleukin 8（IL-8）,tumor necrosis factor α（TNF-α） and so on.At last,the pathological observation of aorta was carried out.Result： Compared with those in model group,the TC,TG,LDL-C,ET,TXB2 and MDA levels and TXB2/PGF1α ratio were reduced,while the HDL-C,the serum SOD,No and 6-keto-PGF1α level were raised in the intervention groups.Although the levels of CRP,IL-6 and IL-8 were lower than model group,there was no obvious effect on the releasing of TNF-α.Conclusion： RSM and FC could inhibit the atherogenesis formation and development,which might be due to regulating the lipid metabolism,enhancing the antioxidation,and reducing the release of inflammatory factors.

关 键 词：动脉粥样硬化 丹参 山楂 

分 类 号：R285.5[医药卫生—中药学]