舟山市贝类副溶血性弧菌污染状况监测及不同来源菌株的毒力基因分析  

Surveillance on the contamination of Vibrio parahaemolyticus and analysis on its virulence genes

作　　者：唐丽华[1] 王虹玲[1] 

机构地区：[1]浙江省舟山市疾病预防控制中心,浙江舟山316021

出　　处：《中国食品卫生杂志》2011年第2期123-126,共4页Chinese Journal of Food Hygiene

基　　金：浙江省医学重点学科建设项目(07-F11);舟山市科技计划项目(07118)

摘　　要：目的监测2009年舟山市海产贝类副溶血性弧菌的污染状况,分析贝源分离株和临床分离株的毒力基因分布情况,了解舟山市副溶血性弧菌的流行规律。方法分春夏秋冬四季对80份舟山市售海产贝类中的副溶血性弧菌进行监测,并对50株贝源分离株和18株部分散发腹泻病例和食物中毒临床分离株进行部分血清分型及tdh、trh、orf8和toxRS/new 4种毒力基因检测。结果春(85%)、夏(80%)、秋(75%)三季的检出率差异无显著性,冬季检出率(10%)明显低于其它季节(P<0.01)。贝源分离株tdh、toxRS/new和orf8基因检测结果均为阴性,只有冬季检出的2株trh阳性。临床分离株中有14株tdh、toxRS/new和orf8基因均为阳性,1株trh阳性,其余3株4种毒力基因均为阴性,说明这18株中有14株(占77.78%)属于大流行株,其中13株(占92.86%)为O3∶K6型,1株为O1∶KUT型。结论春、夏、秋三季海产贝类中副溶血性弧菌的污染率比较高;污染的贝类海产品可能是人感染副溶血性弧菌的主要污染源;一群关系密切,tdh、orf8、toxRS/new均为阳性的O3∶K6克隆群在舟山成优势流行。Objective To survey the contamination of Vibrio parahaemolyticus in shellfish in 2009,and to study the type of virulent genes in isolates from shellfish and clinical samples,in order to find out the epidemic rules of Vibrio parahaemolyticus in Zhoushan.Methods Eighty shellfish samples were collected from Zhoushan markets in four seasons.Fifty isolates from shellfish and 18 isolates from parts of sporadic diarrhea cases and food poisoning patients were serotyped,and the virulent genes,including tdh、trh、orf8 and toxRS/new genes,were detected by PCR.Results The positive rate of Vibrio parahaemolyticus was not significantly different among samples collected in spring（85%）,summer（80%） and autumn（75%）,while the positive rate in winter（10%） was significantly lower than other seasons.The virulent tdh,toxRS/new and orf8 genes were all negative in shellfish isolates,and only virulent trh gene was detected in two isolates collected in winter.The virulent tdh,toxRS/new and orf8 genes were positive in 14 clinical isolates and trh gene was positive in another one clinical isolate.The 4 virulent genes were all negative in the remaining 3 isolates.The results showed that Vibrio parahaemolyticus strains in 14 of 18 clinical isolates（77.78%） were pandemic strains,of which 13 strains（92.86%） were categorized as type O3 ∶ K6,and one was categorized as type O1 ∶ KUT.Conclusion The contamination of Vibrio parahaemolyticus was serious in shellfish in spring,summer and autumn;the shellfish contaminated by Vibrio parahaemolyticus maybe the main source for human diarrhea;A group of Vibrio parahaemolyticus strains with positive O3∶K6 type tdh、orf8 and toxRS/new genes was endemic in Zhoushan.

关 键 词：副溶血性弧菌 毒力基因 监测 

分 类 号：S944[农业科学—水产养殖]