植物有机营养无菌培养试验方法的研究与应用  

STUDY OF STERILE CULTURE METHOD FOR RESEARCHING PLANT ORGANIC NUTRITION AND ITS APPLICATION

作　　者：吴良欢[1] 陶勤南[1] 

机构地区：[1]浙江农业大学,杭州310029

出　　处：《土壤学报》1999年第4期551-558,共8页Acta Pedologica Sinica

基　　金：国家自然科学基金!39430090

摘　　要：本文对植物有机营养研究中无菌培养实验室的结构、功能作了描述，对有机营养无菌水墙液的配制及无菌苗的筛选和培育方法作了检验。用此无菌培养试验法进行的水稻有机氮营养试验证明，水稻能直接吸收根际营养液中的氨基酸。在频繁更换营养液的培养条件下，等氮量（N10mg／L）有机、无机氮对水稻的营养效果依次为甘氨酸态氮（无菌条件）＞谷氨酸态氮（无菌条件）＞铵态氮（无菌条件）＞铵态氮（常规条件），反映出有机、无机氮种类及无菌、常规试验条件对水稻氮素营养的影响。This paper describes the structure and function of plant sterile culture laboratory in details. Some key steps in plant sterile culture such as sterilization of nutrient solution and culture of aseptic seedlings were tested successfully. Results from sterile experiment for rice organic N nutrition showed that amino acid could be directly taken up by plants. The effectiveness of N scarces at equal N concentration (N 10mg/L) for rice followed the order: glycine-N (sterile culture) > glutamic acid-N (sterile culture) > ammonium sulphate-N (sterile culture) > ammonium sulphate-N (conventional culture) under frequent renewal of nutrient solution in the experiment.This indicated clearly the differences among the effciency of various orgnic and inorganic N compounds, and sterile and conventional growth conditions on rice N nutrition.

关 键 词：无菌培养室 氨基酸 水稻 植物有机营养 

分 类 号：Q945.18[生物学—植物学] S511[农业科学—作物学]