蓖麻毒素及其SPDP修饰物对小鼠肝脏GSH含量的影响  

Effect of ricin modified with and without SPDP on GSH levels in mice liver

作　　者：赵英[1] 王文学[1] 董巨莹 蒋宁 

机构地区：[1]第四军医大学军事卫勤统计系毒理学教研室,陕西西安710033

出　　处：《第四军医大学学报》1999年第10期838-840,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金

摘　　要：目的： 观察蓖麻毒素（ＲＴ）修饰前后对小鼠肝脏毒性的影响． 方法： ＲＴ用３－（２－吡啶二巯基）丙酸Ｎ－羟基琥珀酰亚胺酯（ＳＰＤＰ） 一种异型双功能交联剂，进行化学修饰；生成ＲＴ的ＳＰＤＰ衍生物（ＰＤＰ－Ｒ），测定两者在不同剂量和时间对小鼠肝脏还原型谷胱甘肽（ＧＳＨ）含量的影响，以比较两者的毒性差别． 结果： 随着中毒剂量的增加和时间的延长，两者均可使小鼠肝脏ＧＳＨ含量下降（Ｐ＜ ０．０１），但ＲＴ组下降程度较ＰＤＰ－Ｒ组更加明显（Ｐ＜ ０．０１），表明ＰＤＰ－Ｒ对小鼠毒性弱于ＲＴ． 结论： 用ＳＰＤＰ修饰ＲＴ可降低其对小鼠的毒性，这对改进ＲＴ抗癌作用可能是一种有意义的尝试．AIM: To observe the effects of ricin (RT) with and without chemical modification on liver toxicity in mice. METHODS: RT was modified with N succinimidyl 3 (2 pyridyldithio) propionate (SPDP), a heterobifunctional cross linker, and SPDP derivative of RT (PDP R) was obtained. The glutathione (GSH) content in liver of mice, as an index of liver damage, was determined at various doses and time in mice intoxicated with RT and PDP R respectively. RESULTS: The content of GSH decreased with the increaseof the doses or/and the lengthening of time when RT and PDP R were used ( P <0.01), and the decrease of RT group was more significant than that of PDP R group ( P <0.01). CONCLUSION: The liver toxicity of PDP R to mice can be reduced by chemical modification with SPDP, and this may be a new method to decrease side effect while RT is used as an anticancer drug.

关 键 词：蓖麻毒素 化学修饰 GSH SPDP 抗癌作用 

分 类 号：R73－36[医药卫生—肿瘤]