极地适冷菌Pseudoalteromonas sp. QI-1产适冷蛋白酶发酵条件的优化  

Optimization of cold-active protease production by polar psychrotrophic bacterium Pseudoalteromonas sp. QI-1

作　　者：徐国英[1,2] 林学政[1] 王能飞[1] 朱启忠[2] 

机构地区：[1]国家海洋局第一海洋研究所国家海洋局海洋生物活性物质重点实验室,青岛266061 [2]山东大学威海分校海洋学院,威海264209

出　　处：《生物加工过程》2011年第2期5-11,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2007AA091905);中央级公益性科研院所基本科研业务费专项资金项目(2007T11)

摘　　要：对极地适冷菌Pseudoalteromonas sp. QI-1产适冷蛋白酶的发酵条件进行优化。结果表明:菌株QI-1的最适生长和产酶温度均为5℃;最佳接种量为1%;发酵培养基的最适初始pH和最佳装样量分别为5和10%;盐度为2%时对菌株的生长和产酶最为有利;麸皮和醋酸钠分别为最佳N源和C源;添加0.75%酪蛋白时菌株QI-1胞外蛋白酶的活性最高;10 mmol/L Mg2+和0.5%Tween-80有利于产酶。正交试验结果表明:菌株Pseudoalteromonassp. QI-1产蛋白酶较佳培养基配方(g/L)为麸皮5,酵母粉2.5,酪蛋白3,MgCl2.6H2O 3,KCl 1.5;发酵液比酶活为166.20 U/mL,较优化前提高了约56%。The culture conditions of polar psychrotrophic bacterium Pseudoalteromonas sp.QI-1 were optimized for the production of cold-active protease.The optimum fermentation conditions were determined as follows：optimal temperatures for growth and enzyme production were both at 5 ℃,inoculation volume was 1%,and liquid volume was 10% at pH 5.Culture medium for protease production of strain QI-1 was also studied.Results showed that nitrogen source and carbon source preferred by protease production were bran and sodium acetate.2% NaCl,10 mmol/L Mg2＋,0.5% Tween-80 and 0.75% casein were suitable for the enzyme production.The orthogonal experiment design showed that the optimum medium（per liter） consisted of 5 g bran,2.5 g yeast extract,3 g casein,3 g MgCl2·6H2O and 1.5 g KCl.Under the optimum condition,the protease activity was up to 166.20 U/mL and the yield of protease increased about 56%.

关 键 词：适冷菌 发酵 适冷蛋白酶 

分 类 号：Q815[生物学—生物工程]