修饰血红素提高血红蛋白类过氧化物酶活性  

Improving peroxidase-like activity of hemoglobin by recombining hemoglobin using chemically modified heme

作　　者：凌辉生[1] 李阳[1] 封琳[1] 李任强[1] 

机构地区：[1]暨南大学生物工程学系,广州510632

出　　处：《生物加工过程》2011年第2期68-71,共4页Chinese Journal of Bioprocess Engineering

基　　金：暨南大学自然科学基金资助项目(109057640073)

摘　　要：利用苯酚或对羟基联苯对血红蛋白的血红素辅基进行化学修饰,将修饰后的血红素与脱辅基血红蛋白进行重组得到新的血红蛋白。以光吸收扫描分析修饰血红素和重组血红蛋白,证明新的重组血红蛋白构建成功。酶活力测定表明,修饰血红素得到的重组血红蛋白的类过氧化物酶活性都比天然血红蛋白的酶活力高,用对羟基联苯修饰血红素得到的重组血红蛋白的酶活提高明显,约是天然血红蛋白的1.6倍。The phenol or 4-biphenylol was used to modify the heme.The modified hemin group was recombined with apo-hemoglobin to prepare novel artificial hemoglobin.Absorption spectral scan demonstrated that the artificial recombined hemoglobins with modified heme were obtained.Experimental results showed that the peroxidase-like activity of modified hemoglobins was enhanced compared with that of native hemoglobin.And the enzymatic activity of modified hemoglobin with 4-biphenylol modified heme was as about 1.6 folds than that of the native hemoglobin.

关 键 词：化学修饰 血红素 血红蛋白 过氧化物酶 酶活力 

分 类 号：Q554[生物学—生物化学]