肾上腺嗜铬细胞瘤凋亡相关基因bak蛋白表达的意义  

Expression significance of apoptosis related bak gene protein in adrenal pheochromocytoma

作　　者：朱翔[1] 李青 王文亮 孙力 赵宏喜 张献志 

机构地区：[1]第四军医大学

出　　处：《第四军医大学学报》1999年第10期889-891,共3页Journal of the Fourth Military Medical University

摘　　要：目的： 探讨凋亡相关基因ｂａｋ 在人肾上腺嗜铬细胞瘤中的表达及其对肿瘤发生的意义． 方法： 应用免疫组织化学的ＳＡＢＣ方法，和兔抗人Ｂａｋ 多克隆抗体，对２３例肾上腺嗜铬细胞瘤（其中良性１８例，恶性５例）和１０例正常肾上腺组织进行了检测． 结果： Ｂａｋ 在正常肾上腺髓质中不表达，而在良恶性嗜铬细胞瘤中表达，阳性率分别为８９％ 和４０％ ． 在良性肿瘤中Ｂａｋ 表达显著强于恶性肿瘤． 结论： Ｂａｋ 不同程度地参与了良恶性嗜铬细胞瘤中的凋亡调控，对良恶性嗜铬细胞瘤鉴别诊断的意义有待深入研究．AIM: To investigate the expression of bak (bcl 2 homologous antagonist/killer) gene protein in human adrenal pheochromocytoma and the significance of bak expression in tumorigenesis. METHODS: Twenty three paraffin embedded specimens of adrenal pheochromocytoma (18 cases of benign pheochromocytoma and 5 cases of malignant pheochromocytoma) and 10 cases of normal human adrenal gland were investigated by immunohistochemistry (SABC method) for bak gene protein expression. RESULTS: Bak was negative in normal adrenal medulla tissues (10 of 10 cases),but positive in pheochromocytoma (18 of 23 cases, 78%), 89% for benign pheochromocytoma and 40% for malignant. The incidence of Bak positive tumors in benign pheochromocytoma was significantly higher than that in malignant tumors ( P <0.05). CONCLUSION: Though to different degrees, bak is involved in the regulation of apoptosis in benign and malignant pheochrocytoma, which may be a potential means to differentiate the malignant pheochrocytomas from the benign ones.

关 键 词：嗜铬细胞瘤 肾上腺肿瘤 细胞凋亡 BAK基因 

分 类 号：R736.602[医药卫生—肿瘤]