供体与受体移植前预处理延长大鼠移植心脏成活时间  

Prolonged Survival of Rat Cardiac Allograft Resulted by Preconditioning of Donor and Recipient

作　　者：陈凡[1] 宋惠民[2] 徐巨林 李跃华[2] 许复郁[2] 苏润毅 张彦恩[2] 

机构地区：[1]南京铁道医学院附一院胸心外科,南京210009 [2]山东医科大学附一院心外科,济南250012

出　　处：《基础医学与临床》1999年第5期65-69,共5页Basic and Clinical Medicine

摘　　要：目的：用供体品系大鼠皮肤致敏方法及腹腔内异位心脏移植模型研究雷公藤多式供体预处理及供体脾细胞受体胸腹内注射诱导免疫耐受对移植心脏存活影响及二种方法的协同效果。方法：SD大鼠作供体，Wistar大鼠作受体，所有受体在心脏移植前均无用供体皮肤致敏，A组作单纯心脏移植，B组作经供体预处理后的供心移植，C组用供体脾细胞胸腺内注射诱导耐受后作心脏移植，D组考察供受体两种预处理方法的协同效果。结果：各处理组供心存活时间均校对照组明显延长，C，D二组病检见淋巴细胞浸润及心肌坏死明显减轻，但微血管病变较严重。结论：两种方法均能明显延长移植供心成活时间，并有良好的协同效果，经胸腺途径诱导的耐受能明显抑制细胞免疫，但对急性血管排斥没有预防作用。Objective: Using donor skin sensitized recipients and illtraabdominal heterotopic cardiac transplantprocedure, we investigated the effect of donor pretreatment with tripterygium polyglycoside andrecipient pretreatment with intrathymic inoculation of donor splenocytes and their synergistic effectson the cardiac allograft survival. Methods: SD rat used as skin and heart donor and Wistar rat used asrecipient. All recipiets were sensitized with donor skin transplantation before heart transplantation(HT). There was single HT only in group A and donor pretreated HT in group B. After allogenicsplenocytes being inoculated in group C the heart was transplanted. In group D the two pretreat-ments were combined. ResuIts: The mean survival time ofcardiac allografts was much prolonged ingroup B, C and D in comparing with those in control group A. Microscopic examination showedthat the cell mediated rejection was much alleviated in group C and D, but the vascuIar rejection wasaggravated. Conclusion: Both of the method could evidently promote survival of cardiac allografts andact synergistically. Tolerance induction could obviosly depress the cell mediated immunity, but seemedto show no effect on prevention of the vessels injury caused by acute vascular rejection.

关 键 词：雷公藤多甙 移植前预处理 心脏移植 免疫耐受 

分 类 号：R654.2[医药卫生—外科学]