动物源性食品中群勃龙ELISA检测技术的研究与应用  

Research and Application on ELISA for Detection of Trenbolone in Animal Derived Food

作　　者：冯才伟 何方洋[2] 周德刚[2] 聂雯莹[2] 

机构地区：[1]北京望尔生物技术有限公司,北京102206 [2]北京市兽药监察所,北京100012

出　　处：《畜牧与饲料科学》2011年第1期105-108,共4页Animal Husbandry and Feed Science

基　　金：国家863计划(2010AA10Z402)

摘　　要：采用琥珀酸酐法对群勃龙进行化学修饰,合成半抗原,免疫动物,制备了特异性强的群勃龙单克隆抗体。建立了群勃龙ELISA检测方法,其灵敏度为0.05μg/L,对群勃龙的交叉反应率为100%,对去氢表睾酮和睾酮交叉反应率小于1%,19-去甲睾酮交叉反应率小于4.5%。以2.5,5.0,10.0μg/kg添加水平的牛肉样本回收率范围为78.4%~105.6%,变异系数小于15%。该方法的建立为群勃龙残留检测提供了可靠的分析检测手段。Trenbolone was chemically modified by succinic anhydride.Its hapten was synthesized and used to immunize animals,and specific monoclonal antibody against trenbolone was prepared.ELISA for detecting trenbolone was developed.Its sensitivity was 0.05 μg/L.Its cross-reactivity against trenbolone was 100%;that against boldenone and testosterone was less than 1%;and that against 19-nortestosterone was less than 4.5%.The recovery rate of beef samples added trenbolone at the concentrations of 2.5,5.0 and 10.0 ug/kg was 78.4%-105.6%.The coefficient of variation was less than 15%.This method is reliable to detect trenbolone residues.

关 键 词：群勃龙 ELISA 牛肉 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]