鹅肠道纤维素分解菌的分离鉴定及其产酶条件的优化  

Isolation,Identification and Optimization of Enzyme Production of Cellulolytic Bacteria from the Goose Gut

作　　者：高云航[1,2] 张喜宏[1] 刘佳丽[1] 战利[1] 李长亮 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]农业部生态重点开放实验室,广州510900 [3]敦化市林业局,敦化133700

出　　处：《动物营养学报》2011年第3期466-472,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家科技支撑计划(2007BAD89B06-31);农业部生态重点开放实验室开放课题(2009k22);吉林省自然科学基金项目(20101578)

摘　　要：本研究拟在鹅肠道筛选1株高效降解纤维素的菌株,并对该菌株的产酶条件进行研究。通过对鹅肠道菌群进行富集培养、分离纯化,利用刚果红法(初筛)和摇瓶法(复筛)得到1株产酶活较高的纤维素分解菌E2。经16S rDNA核苷酸序列比对分析表明,该菌株为芽孢杆菌属的短小芽孢杆菌。单因素试验得出菌株E2的产酶最适碳源为玉米粉,最适氮源为牛肉膏和蛋白胨混合物,最适初始pH为6.0,最适发酵温度为42℃,最适发酵时间为48 h。A strain of highly cellulolytic bacteria was obtained from goose gut,and the conditions in enzyme production have been studied in this study.The strain of highly cellulolytic bacterial E2 has been isolated by the Congo red（primary screening） and shaking flask method（secondary screening）.The 16S rDNA sequence analysis indicated that strain E2 was Bacillus pumilus sp.On the basis of single factor experiment,the optimal carbon source,nitrogen source,initial pH,fermentation temperature and fermentation time of strain E2 for enzyme production were maizena,beef extract and peptone mixture,6.0,42 ℃,and 48 h,respectively.

关 键 词：鹅 纤维素分解菌 分离 鉴定 产酶条件 优化 

分 类 号：S835[农业科学—畜牧学]