Total DNA of Glycyrrhiza uralensis transformed into Hansenula anomala by ion implantation:Preparing Glycyrrhizic acid in recombined yeasts  

作　　者：Jin Xiang Lue Jie Ma Yuan Mao Peihong 

机构地区：[1]Xinjiang Univ, Coll Phys & Technol, Ctr Ion Beam Biotechnol, Urumqi 830008, Peoples R China [2]Xinjiang Univ, Coll Resource & Environm Sci, Urumqi 830046, Peoples R China [3]Nanjing Univ Technol, Coll Life Sci & Pharm, Nanjing 210009, Peoples R China

出　　处：《Nuclear Science and Techniques》2010年第6期347-351,共5页核技术（英文）

基　　金：Supported by National Natural Science Foundation of China(Grant No.30560182,30960006)

摘　　要：Glycyrrhizic acid(GA) in Glycyrrhiza uralensis(G.uralensis) is physiologically active.In this study,the total DNA of wild G.uralensis was randomly transformed into Hansenula anomala by implantation of low-energy Ar^+ and N^+,to produce five recombinant yeast strains relating to biological synthesis of the GA or Glycyrrhetinic acid (GAs).After culturing in liquid medium for 96 h,the resultant GA,18α-GAs and 18β-Gas were determined by reversed-phase high performance liquid chromatography(RP-HPLC),and the corresponding concentrations were 114.49,0.56,and 0.81 mg·L^(-1).After one hundred primers were analyzed with random amplified polymorphic DNA (RAPD),the seven different DNA fragments were produced by the N7059 strain of recombined yeasts,and,the polymerase chain reaction(PCR) verified that one of them came from the genome of G.uralensis,indicating a successful transfer of genetic information by ion implantation.Glycyrrhizic acid（GA） in Glycyrrhiza uralensis（G.uralensis） is physiologically active.In this study,the total DNA of wild G.uralensis was randomly transformed into Hansenula anomala by implantation of low-energy Ar~＋ and N~＋,to produce five recombinant yeast strains relating to biological synthesis of the GA or Glycyrrhetinic acid （GAs）.After culturing in liquid medium for 96 h,the resultant GA,18α-GAs and 18β-Gas were determined by reversed-phase high performance liquid chromatography（RP-HPLC）,and the corresponding concentrations were 114.49,0.56,and 0.81 mg·L^（-1）.After one hundred primers were analyzed with random amplified polymorphic DNA （RAPD）,the seven different DNA fragments were produced by the N7059 strain of recombined yeasts,and,the polymerase chain reaction（PCR） verified that one of them came from the genome of G.uralensis,indicating a successful transfer of genetic information by ion implantation.

关 键 词：异常汉逊酵母 DNA转化 重组酵母 离子注入 甘草酸 乌拉尔甘草 随机扩增多态性DNA 反相高效液相色谱法 

分 类 号：Q959.468[生物学—动物学] TQ926[轻工技术与工程—发酵工程]