SGC7901和SGC7901/ADR细胞中S100A6蛋白及mRNA的表达  

Expression of S100A6 protein and mRNA in SGC7901 and SGC7901/ADR cells

作　　者：李晖[1] 陈丹[2] 唐颖[3] 王丽芬[3] 关宏伟[2] 

机构地区：[1]郑州大学西亚斯国际学院护理学院,新郑451150 [2]大连医科大学第一临床学院病理科,大连116011 [3]大连医科大学第二临床学院病理科,大连116011

出　　处：《郑州大学学报（医学版）》2010年第6期934-936,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：辽宁省教育厅基金资助项目20272274

摘　　要：目的:探讨S100A6与胃癌多药耐药的关系。方法:常规培养人胃癌细胞SGC7901及其耐药细胞株SGC7901/ADR,在SGC7901/ADR细胞培养基中加入阿霉素(0.375mg/L)维持耐药。取对数生长期的SGC7901和SGC7901/ADR细胞,分别采用免疫细胞化学方法、Westernblot方法和RT-PCR检测S100A6蛋白及mRNA的表达情况。结果:免疫细胞化学染色结果显示S100A6在SGC7901细胞中的阳性染色强度高于SGC7901/ADR细胞;Westernblot结果显示SGC7901细胞中S100A6蛋白的相对表达量(4.356±0.759)高于SGC7901/ADR细胞的(2.243±0.089)(t=4.788,P=0.039);RT-PCR结果显示SGC7901细胞中S100A6mRNA的相对表达量(0.659±0.017)与SGC7901/ADR的(0.582±0.060)相比,差异无统计学意义(t=2.125,P=0.150)。结论:S100A6蛋白在SGC7901和SGC7901/ADR细胞中的差异性表达可能与细胞多药耐药有关;这种差异表达是翻译后事件。Aim：To study the role of S100A6 in the development of multidrug resistance（MDR） in human gastric carcinoma.Methods：Human gastric cancer cell line SGC7901 and its drug-resistant cell line SGC7901/ADR were cultured in vitro,and adriamycin（0.375 mg/L） was added into SGC7901/ADR for its drug-resistance.SGC7901 and SGC7901/ADR growing in logarithmic phase were collected,and immunocytochemistry（SP-method） and Western blot were used to observe the expression of S100A6 protein and Semi-quantity RT-PCR technology was adopted to detect the expression of S100A6 mRNA.Results： Immunocytochemistry staining showed that the positive staining intensity for S100A6 in SGC7901 was significantly higher than that in SGC7901/ADR;the expression level of S100A6 protein in SGC7901（4.356±0.759） was higher than that in SGC7901/ADR（2.243±0.089）（t=4.788,P=0.039）;the expression level of S100A6 mRNA in SGC7901（0.659±0.017） did not differ from that in SGC7901/ADR（0.582±0.060）（t=2.125,P=0.150）.Conclusion：The differential expression of S100A6 in SGC7901 and SGC7901/ADR may be associated with MDR of SGC7901/ADR,which begins from the post-translation level.

关 键 词：S100A6 多药耐药 SGC7901细胞 SGC7901/ADR细胞 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]