常染色体短串联重复序列基因座在河南汉族人群亲子鉴定中的应用  

Application of 24 auto-chromosome STR loci in paternity identification of Henan Han population

作　　者：陈辉[1] 徐朝阳[1] 李晓文[1] 郑红[1] 程晓丽[1] 贺颖[1] 宋国英[1] 刘华[1] 

机构地区：[1]郑州大学基础医学院细胞生物学与医学遗传学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2010年第6期983-985,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省自然科学基础研究基金资助项目0111022300

摘　　要：目的:分析24个常染色体短串联重复序列(STR)在河南省汉族群体的遗传多态性及其在亲子鉴定案例中的应用价值。方法:应用多位点复合PCR扩增技术及聚丙烯酰胺凝胶电泳分型技术,对773例亲子鉴定案例进行分析。结果:D7S3048、D18S51、D8S1179、D8S1132、D11S2368、D2S1338、D4S2639和D12S391的个体识别率≥0.94,观察杂合度≥0.79,多态性信息含量≥0.80,且这些位点参与联合排除父权率均在40%以上。结论:短串联重复序列D7S3048、D18S51、D8S1179、D8S1132、D11S2368、D2S1338、D4S2639和D12S391可作为亲子鉴定的首选基因座,在法医学应用和群体遗传学研究中有较高的价值。Aim：To analyze the application value and genetic polymorphism of 24 auto-chromosome short tandem repeats（STR） in paternity identification of Henan Han population.Methods：DNA samples of 773 cases from forensic test were extracted, then 24 STR loci were amplified and electrophoresed on denaturalization polyacrylamide gel for allele genotypes analysis.Results：Among 24 STR loci, there were 8 loci（D7S3048,D18S51,D8S1179,D8S1132,D11S2368,D2S1338,D4S2639,and D12S391） with DP≥0.94, Ho≥0.79,PIC≥0.80,and their ratios taking part in excluding paternity were all above 40%.Conclusion： These loci could be the first chosen loci in paternity test and population genetic study.

关 键 词：短串联重复序列 遗传多态性 亲子鉴定 

分 类 号：R394.3[医药卫生—医学遗传学]