差不嘎蒿自然种群的遗传多样性与遗传分化  

Genetic Diversity and Differentiation of Five Natural Populations of Artemisia halodendron

作　　者：王铁娟[1] 李伟琼[1] 张淑艳[1] 孟翔飞[1] 

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022

出　　处：《林业科学》2010年第12期171-175,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(40561004)

摘　　要：差不嘎蒿（Artemisia halodendron）也称盐蒿,是菊科（Compositae）蒿属（Artemisia）的多年生半灌木,为中旱生沙生植物。可生长于流动沙丘的背风坡、半流动沙丘、半固定沙地、固定沙地和干旱坡地,在半流动、半固定沙地优势作用明显（李进,1991;Artemisia halodendron is a dominant species of desert vegetation and an important sand-fixed plant in northeastern China.In this study,inter-simple sequence repeat（ISSR） markers were applied to investigate the genetic diversity and differentiation of five natural populations of A.halodendron.A total of 230 bands were amplified by 12 ISSR primers,of which 226 were polymorphic.Percentage of polymorphic bands（PPB） and Shannon＇s phenotypic diversity index（Ho） were respectively 98.26% and 0.329 6 at the species level,and 61.22% and 0.285 3 at the population level,showing a high level of genetic diversity.Analysis of molecular variance（AMOVA） revealed that 90.1% of the total genetic diversity occurred within populations.Based on the clustering analysis（UPGMA）,the five populations were divided into two groups： the Hulunbuir Sandy Land group and the Horqin Sandy Land group.The Mantel test showed that genetic distance was significantly positively correlated with geographical distance.

关 键 词：差不嘎蒿 ISSR 遗传多样性 遗传分化 

分 类 号：S718.46[农业科学—林学] S718.54