瑞芬太尼对兔心肌缺血再灌注时NF-κB活性的影响  

Effects of remifentanil on NF-kB activity in a rabbit model of myocardial ischemia/reperfusion injury

作　　者：王伟[1] 杨新园[2] 赵新京[1] 

机构地区：[1]西安交通大学医学院第一附属医院麻醉科,710061 [2]西安交通大学医学院第一附属医院妇产科,710061

出　　处：《中华麻醉学杂志》2010年第11期1364-1366,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：陕西省科技攻关项目（2008K13-02）

摘　　要：目的 探讨瑞芬太尼对兔心肌缺血再灌注时NF-κB活性的影响.方法 健康成年新西兰大白兔50只,月龄2～3月,体重2.0～2.5 kg,随机分为5组（n=10）：假手术组（S组）左冠状动脉前降支只穿线不结扎;缺血再灌注组（IR组）冠状动脉前降支穿线结扎30 min,再灌注120 min;S组和IR组在缺血前10 min时颈外静脉输注生理盐水5 ml·kg-1·h-1至再灌注120 min;低、中、高剂量瑞芬太尼组（L组、M组、H组）缺血前10 min时颈外静脉输注瑞芬太尼1、5、10μg·kg-1·min-1至再灌注120 min,余处理同IR组.再灌注120 min时处死动物取心脏,采用Western blot法测定心肌细胞核NF-κB的表达水平,以反映其活性,观察心肌组织病理学结果.结果 与S组比较,其余各组NF-κB活性升高（P＜0.05）;与IR组比较,L组、M组和H组NF-κB活性降低（P＜0.05）,L组、M组和H组NF-κ：B活性依次降低（P＜0.05）;病理学结果显示：L组、M组和H组心肌缺血再灌注损伤程度较IR组减轻,且随剂量增加,损伤逐渐减轻.结论 静脉输注瑞芬太尼可通过降低NF-κB活性减轻兔心肌缺血再灌注损伤,其效应呈剂量依赖性.Objective To investigate the effects of remifentanil on NF-κB activity in a rabbit model of myocardial ischemia/reperfusion （I/R） injury. Methods Fifty New Zealand white rabbits were randomly divided into 5 groups （n = 10 each）： sham operation group （group S）; group I/R; low dose of remifentanil group （group L）; median dose of remifentanil group （group M）; high dose of remifentanil group （group H）. In group I/R, L,M and H, myocardial I/R was produced by occlusion of left anterior descending artery for 30 min followed by 120 min reperfusion.In group S and I/R,10 min before ischemia,normal saline was infused at 5 ml·l·h-1 via the external jugular vein until the end of 120 min reperfusion. In group L, M and H, remifentanil was infused at 1, 5 and 10ug·kg·min-1 respectively 10 min before ischemia until the end of 120 min reperfusion,and the other procedures were the same as those in group I/R. The myocardial tissues were taken at the end of 120 min reperfusion for determination of NF-κB expression which was used to reflect the activity of NF-κB and microscopic examination. Results The activity of NF-κB was significantly higher in group I/R, L, M and H than in group S. The activity of NF-κB was gradually decreased with the increase in the dose of remifentanil in group L, M and H compared with group I/R. The microscopic examination showed that remifentanil significantly attenuated I/R-induced injury in a dose-dependent manner. Conclusion Infusion of remifentanil reduces myocardial I/R injury through decreasing the activity of NF-κB in a doee-dependent manner.

关 键 词：哌啶类 心肌再灌注损伤 NF-KB 

分 类 号：R965[医药卫生—药理学]