κ基因重排在霍奇金淋巴瘤H-RS细胞性质中的研究  

The research of the nature of H-RS cells in Hodgkin′s lymphoma with detecting the Igκ light chain gene rearrangements

作　　者：苏俊[1] 刘勇[1] 邓飞[1] 

机构地区：[1]遵义医学院附属医院病理科,563003

出　　处：《实用医学杂志》2010年第24期4469-4472,共4页The Journal of Practical Medicine

基　　金：国家教育部青年骨干教师项目(编号:黔教高发[2000]371号)

摘　　要：目的:采用直接原位PCR法,对霍奇金淋巴瘤的恶性成分——H/R-S细胞的克隆性及其与背景淋巴细胞的相关性进行研究。方法:采用直接原位PCR法,5′-生物素标记的Vκ/Jκ1124、Vκ/Jκ2124、Vκ/Jκ、Vκ/334Jκ124、Vκ/Jκ、Vκ/Jκ556混合等6对κ家族特异性引物对3例霍奇金淋巴瘤组织H/R-S细胞的κ轻链基因重排情况进行检测。结果:(1)1例结节硬化型霍奇金淋巴瘤(NSHL)组织中,部分H/R-S细胞重复出现Vκ/Jκ33基因重排,亦有部分H/R-S细胞重复出现Vκ/Jκ基因重排,且阳性信号分别重复出现于其周围部分背景1124淋巴细胞核内,少数H/R-S细胞未检测出κ基因重排;(2)2例混合细胞型霍奇金淋巴瘤(MCHL)组织中,所有H/R-S细胞均未检测出κ轻链基因重排。结论:(1)本例NSHL的H/R-S细胞可能为多克隆性增生,且H/R-S细胞与其周围部分背景淋巴细胞间可能存在克隆相关性,即可能来自同一个B细胞克隆;(2)2例MCHL的H/R-S细胞发生了λ基因重排或是采用的κ家族特异性引物法覆盖其Igκ轻链的基因重排方式。Objective To study the cloning of H/R-S cells in Hodgkin′s lymphoma （HL） and the relationships between H/R-S cells and its surrounding background lymphocytes by direct in situ PCR. Methods Selected 6 pairs of 5′ bio-labeled κ gene-family-specific primers： Vκ1/Jκ1 2 4, Vκ2/Jκ1 2 4, Vκ3/Jκ3, Vκ4/Jκ1 2 4, Vκ5/Jκ5, Vκ6/Jκ混合, detected the light chain gene rearrangements in 3 cases of HL by the method of direct in situ PCR. Results （1） Vκ3/Jκ3 and Vκ1/Jκ1 2 4 gene rearrangements were observed in the same case of Hodgkin′s lymphoma nodular sclerosing type （NSHL）, and the positive signals were also found in it′s surrounding background small lymphocytes. But the κ gene rearrangements were not observed in some H/R-S cells of NSHL. （2）κ gene rearrangements were not observed in all H/R-S cells of the cases of Hodgkin′s lymphoma mixed cellularity type （MCHL）. Conclusions （1） The case of NSHL might originate from different B cells clone, the H/R-S cells and it′s surrounding background lymphocytes might originate from the same B cell clone. （2） The two cases of MCHL take the λ gene rearrangements or the six pairs of κ gene-family-specific primers can not cover its Igκ gene rearrangements.

关 键 词：霍奇金病 直接原位PCR法 H/R-S细胞 κ家族特异性引物 基因重排 

分 类 号：R733.1[医药卫生—肿瘤]