Expression of the Calbindin-d28k Protein and Gene in Human Ovary  

作　　者：Duo XU Wei-jie ZHU 

机构地区：[1]Institute of Reproductive Immunology,Jinan University,Guangzhou 510632,China

出　　处：《Journal of Reproduction and Contraception》2010年第4期213-218,共6页生殖与避孕(英文版)

摘　　要：Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods. Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. From primordial follicle, primary follicle to secondary follicle, increased CaBP- d28k levels were showed (P〈0. 05), and the CaBP-d28k expression in secondary follicle had the highest level. Conclusion Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods. Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. From primordial follicle, primary follicle to secondary follicle, increased CaBP- d28k levels were showed (P〈0. 05), and the CaBP-d28k expression in secondary follicle had the highest level. Conclusion Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.

关 键 词：HUMAN OVARY CALBINDIN-D28K 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]