胃肠道间质瘤中DOG1和nestin的表达及意义  

Expression of DOG1 and nestin and the clinical significance in gastrointestinal stromal tumors

作　　者：蒋春莲[1] 黄文斌[1] 王劲松[1] 李俐[1] 杨小兵[1] 赵建华[1] 

机构地区：[1]南京医科大学附属南京第一医院病理科,南京210006

出　　处：《国际病理科学与临床杂志》2010年第6期465-468,共4页Journal of International Pathology and Clinical Medicine

摘　　要：目的:探讨DOG1和nestin在胃肠道间质瘤(gastrointestinal stromal tumor,GIST)中的表达及其诊断价值。方法:采用免疫组织化学EnVision法分别检测25例GIST、5例平滑肌瘤和5例神经鞘瘤中DOG1和nestin的表达。结果:DOG1和nestin在GIST中的阳性表达率分别为100%和88%,而CD117和CD34在GIST中的阳性表达率分别为84%和64%。GIST中DOG1和nestin的表达与肿瘤部位、肿瘤大小、危险度分级和组织学形态均无关(P>0.05)。5例平滑肌瘤中DOG1和nestin均阴性表达,DOG1和nestin在神经鞘瘤中的阳性表达率分别为0和100%。DOG1,nestin,CD117和CD34联合检测阳性率为48%。结论:DOG1在诊断GIST中具有敏感性和特异性,而nestin则是GIST诊断中比较敏感的一种标志物,但特异性差。DOG1,nestin,CD117和CD34联合检测可提高GIST的正确诊断。Objective To study the expression of DOG1 and nestin in gastrointestinal stromal tumors（GISTs） and determine the value for the diagnosis of GISTs.Methods The expression of DOG1 and nestin in 25 cases of GISTs,5 cases of leiomyoma,and 5 cases of neurinoma was detected by EnVision immunohistochemical technique.Results The positive rates for the expression of DOG1 and nestin in GISTs were 100% and 88%,respectively,whereas the positive rates for the expression of CD117 and CD34 in GISTs were 84% and 64%,respectively.The expression of DOG1 and nestin in GISTs was not related to the location,size,grade,or histological subtype of GISTs（P0.05）.The expression of DOG1 and nestin in leiomyoma was negative.The positive rates for the expression of DOG1 and nestion in neurinoma were 0 and 100%,respectively.Conclusion DOG1 is a sensitive and specific marker in the diagnosis of GISTs.Nestin is also sensitive but less specific for GISTs.Combined detection of DOG1,nestin,CD117,and CD34 may improve the diagnosis of GIST.

关 键 词：胃肠道间质瘤 DOG1 NESTIN CD117 CD34 

分 类 号：R735[医药卫生—肿瘤]