凤眼草抗肿瘤活性成分的分离与鉴定  

Studies on the anti-tumor constituents of fruits of Ailanthus altissima (Mill) Swingle

作　　者：赵春超[1] 张彪[1] 范菊娣[2] 邵建华[1] 

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009 [2]贵阳医学院药学院,贵阳550004

出　　处：《扬州大学学报（自然科学版）》2010年第4期39-41,共3页Journal of Yangzhou University：Natural Science Edition

摘　　要：对苦木科臭椿属植物臭椿(Ailanthus altissima(Mill)Swingle)的果实即凤眼草的提取物进行抗肿瘤活性研究.经反复纯化制备化合物,并根据化合物的理化性质分析、波谱解析、对照品比较及与文献报道对比鉴定化合物的化学结构;采用四甲基偶氮唑盐(MTT)法对粗提物和单体化合物进行抗肿瘤活性的测试.分离并鉴定出4个化合物,分别为shinjulactone A(1),shinjuglycoside B(2),5-hydroxymethylfural-dehyde(3),protocatechuic acid(4),化合物3,4为从该属植物中首次分离得到.活性筛选结果表明,凤眼草水提取物的大孔树脂60%乙醇洗脱物(Ⅰ)对人神经胶质肿瘤细胞株U87,95%乙醇浸提物的乙酸乙酯萃取物(Ⅱ)对人神经胶质肿瘤细胞株SF188,化合物1和2对以上两种细胞株,均有较好的抑制作用.The author investigates the anti-tumor constituents of fruits of Ailanthus altissima（Mill） Swingle.Four compounds are isolated by chromatography and identified by spectral data.The anti-tumor activities of two of them and the extracts of the fruits of Ailanthus altissima（Mill） Swingle are evaluated by MTT.The compounds obtained are identified as shinjulactone A（1）,shinjuglycoside B（2）,5-hydroxymethylfuraldehyde（3）,protocatechuic acid（4）.Compounds 3 and 4 are isolated from this genus for the first time.The anti-tumor results demonstrate that compounds 1 and 2,together with extracts Ⅰ（the extract with water of fruits of Ailanthus altissima chromatographed on HPD-100 resin and eluted 60% ethanol） and Ⅱ（the EtOAc extract of ethanolic extract of fruits of Ailanthus altissima）,exhibit moderate antiproliferative activity.

关 键 词：臭椿 凤眼草 化学成分 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学] R962[医药卫生—中医学]