5-脂氧合酶在梗阻性黄疸大鼠肝组织中的表达  

Expression of 5-lipoxygenase in liver of obstructive jaundice rats

作　　者：蔡军[1] 宋超[1] 徐三荣[2] 

机构地区：[1]江苏大学临床医学院,江苏镇江212001 [2]江苏大学附属医院普外科,江苏镇江212001

出　　处：《中国现代医学杂志》2010年第24期3698-3702,3706,共6页China Journal of Modern Medicine

摘　　要：目的研究5-脂氧合酶(5-lipoxygenase,5-LOX)在梗阻性黄疸大鼠(obstructive jaundice,OJ)肝组织中的表达。方法将48只雄性SD大鼠随机分为3组:假手术组(Sham组)、胆管结扎组(BDL)和AA-861干预组(AA-861组)。分别于术后第7天、14天处死大鼠,鲎试剂测定血浆内毒素含量,用免疫组织化学法检测肝组织5-LOX的表达,ELISA法检测大鼠血清LTB4及TNF-ɑ、IL-6水平;肝组织匀浆检测丙二醛(MDA)、超氧化物歧化酶(SOD)的含量,全自动生化分析仪测定血浆ALT、AST水平。结果 Sham组5-LOX轻微表达,BDL组明显表达,AA-861组各时相点相比,BDL组5-LOX表达显著降低,BDL、AA-861组较Sham组血浆内毒素、LTB4、TNF-α、IL-6、ALT、AST水平明显升高,AA-861组较BDL组每个时相点LTB4、TNF-α、ALT、IL-6、ALT、AST水平明显降低,差异有显著性,AA-861组较BDL组肝组织MDA含量明显减少;SOD含量明显增高。结论梗阻性黄疸时,5-LOX表达明显增加,使用AA-861后,5-LOX表达明显减少,肝损伤明显减轻。【Objective】To investigate the expression of 5-lipoxygenase in the liver during obstructive jaundice.【Methods】Forty eight male Sprague-Dawley rats were randomly divided into three groups： sham-operation group（sham）,bile duct-ligation group（BDL）,and bile duct-ligation plus AA-861 group（AA-861）.The rats were sacrificed at 7 and 14 days after operation.The levels of plasma endotoxin were measured using limulus test;Immunohistochemitry were used to detect the expression of 5-lipoxygenase of liver.The serum levels of LTB4 and TNF-ɑ,IL-6 were analyzed by enzyme-linked immunosorbent assay（ELISA）.To test the content of MDA and SOD of the liver tissue by homogenate,ALT and AST were test by Automatic biochemical analyzer.【Results】Expression of 5-LOX was significantly lower in sham group,the expression of 5-LOX was obviously increased in BDL group,and the expression of 5-lipoxygenase was significantly lower at each time point in AA-861 group than in BDL group.The serum levels of LTB4,TNF-ɑ,IL-6,ALT,AST and endotoxin were significantly lower in sham group than BDL and AA-861 group,The serum levels of LTB4,TNF-ɑ,IL-6,ALT,AST were significantly lower at each time point in AA-861 group than in BDL group,the liver tissue levels of MDA was significantly lower in AA-861 group than in BDL group,the liver tissue levels of SOD was significantly higher in AA-861 group than in BDL.【Conclusion】Expression of 5-LOX is significantly higher during obstructive jaundice,AA-861 may protect against the liver injury in rat with obstructive jaundice through reducing the expression of 5-LOX in liver.

关 键 词：梗阻性黄疸 5-脂氧合酶 LTB4 AA-861 内毒素性肝损伤 

分 类 号：R-333.4[医药卫生] R575