乳牙釉质表面的脱矿与再矿化的体外研究  

Study of de- and re-mineralization on the enamel surface of primary teeth in vitro

作　　者：汪俊[1] 毛培芬[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔儿童预防科,上海市口腔医学研究所·上海市口腔医学重点实验室,上海200011

出　　处：《口腔材料器械杂志》2010年第3期129-132,共4页Chinese Journal of Dental Materials and Devices

基　　金：上海市科学技术委员会科研计划项目(074119644;09DZ2272100);上海重点学科建设项目资助(S30206)

摘　　要：目的运用共聚焦激光扫描显微镜(CLSM)评价离体乳牙釉质表面的脱矿与再矿化行为。方法临床选取无龋健康的上颌乳切牙36颗,于唇面中央开3个1 mm×1 mm的窗a、b、c。开窗面a进行酸蚀、再矿化处理,b进行酸蚀处理,c不进行任何处理。将36个样本随机分为3组,组1、2、3的开窗面a在酸蚀后分别进行再矿化24h、48h及72h。然后运用CLSM技术测量开窗面a、b、c的总荧光量(TF)、平均荧光量(AF)及荧光面积(A)。对正态分布资料,用t检验进行统计分析,对非正态分布资料,则使用非参数分析,统计学差异指标为P<0.05。结果开窗面b、c间TF、AF、A差异均有显著性(P<0.05),各组别(不同矿化时间)开窗面a、b间TF、AF及A差异均有显著性(P<0.05),开窗面a、c间TF、AF及A差异均有显著性(P<0.05)。结论 CLSM能有效地评价乳牙釉质的脱矿与再矿化行为。Objective To study the behavior of de- and re-mineralization on the enamel surface of deciduous teeth in vitro using Confocal Laser Scanning Microscope（CLSM）.Methods 36 healthy maxillary primary incisors were recruited in this study.3 windows（1×1 mm^2 ）,named as a,b and c,were made on the labial enamel surface.Window a was etched and re-mineralized,window b was etched only,and window c was left alone.36 samples were randomly assigned to 3 groups. In group 1,2 and 3,window a was re-mineralized 24h,48h,72h respectively.Afterwards,TF,AF and A in window a,b,c were measured using CLSM.T-test was applied to analyze normal distribution data,and non-parameter analysis was used for nonnormal distribution data.The significance level was P0.05.Results The difference of TF,AF and A between window b and c was significant（P0.05）.In each group（different remineralization time）,the difference of TF,AF and A between window a and b was significant,and so is the difference between window a and c（P0.05）.Conclusions CLSM may be applied to the study of de- and re-mineralization on the enamel surface of primary teeth in vitro.

关 键 词：脱矿 再矿化 共聚焦激光扫描显微镜 乳牙 釉质表面 

分 类 号：R781.1[医药卫生—口腔医学]