中医脾脏病理生理状态下唾液的代谢组研究  

Metabonomics Research of Saliva in the Physiopathological State of Chinese Medicine Spleen Based on NMR

作　　者：赵晓山[1] 余克强[1] 孙晓敏[1] 罗云坚[2] 杨洪玲 聂晓莉[1] 李玉萍[1] 刘艳艳[1] 罗仁[1] 

机构地区：[1]南方医科大学南方医院中医科,广东广州510515 [2]广州中医药大学附属广东省中医院内二科,广东广州510105 [3]广州市妇幼保健医院检验科,广东广州510180

出　　处：《四川中医》2011年第3期43-45,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金课题(项目编号:30500642);中国博士后科学基金课题(项目编号:2005037577);广东省自然科学基金课题(项目编号:5300058)

摘　　要：目的:研究中医脾脏病理生理状态下唾液的代谢组变化。方法:采用病证结合的方法,选择慢性胃炎、消化性溃疡病以及亚健康状态等脾气虚证、湿热蕴脾证患者为实验组(即"脾病"组),以正常人作为正常对照组(即"脾未病"组)。采用标准化方法收集全唾液,应用核磁共振仪(NMR)测定唾液中的代谢物组。结果:脾脏在病理生理状态下唾液中1H NMR结果显示,实验各组唾液1H NMR谱的主成分积分值集中分布于椭圆形散点图(95%置信区内)的3个区域,脾气虚证组、湿热蕴脾组与正常对照组无明显交叉和重叠。进一步对1H NMR谱变化值影响较大的两个主成分PC1和PC2的分析表明,正常对照组唾液中主要是葡萄糖(3.82 ppm)、N-乙酰糖蛋白(2.02ppm)、比咯氨酸(2.06 ppm)、乙醇(1.18 ppm)、山梨醇(3.66 ppm)、羟脯氨酸(2.34 ppm)等物质含量相对较高;湿热蕴脾组唾液中主要是乙酸(1.9 ppm),丙酸盐(1.06 ppm和2.18 ppm)、牛磺酸(3.42 ppm)等物质含量相对较高;脾气虚证组唾液中主要是谷氨酰胺(2.14 ppm)、蔗糖(4.22 ppm)、乳酸盐(4.1 ppm和4.14 ppm)、苯丙氨酸(3.26 ppm)等物质含量相对较高。结论:脾脏虚实状态下(脾气虚证、湿热蕴脾证)唾液中有部分代谢物发生了变化,其可以作为脾气虚证、湿热蕴脾证的辨证参考指标,这揭示了中医"脾在液为涎"理论的科学性。Objective：Metabonomics research of saliva in the physiopathological state of Chinese medicine spleen.Methods：With the method of combining disease identification and differentiation of symptom-complex,people suffered from the syndrome of deficiency of spleen-Qi and the syndrome of damp-heat stagnating in the spleen of chronic gastritis or peptic ulcer disease and sub-health,accepted as the experimental group.Normal people were as a normal control group.Saliva samples were collected by standardized methods.The biochemical indicator of saliva were detected by the nuclear magnetic resonance（NMR） instrument.Results：The principal component value of saliva 1H NMR of experiment Showed that every group distributes on three without overlapping and crossing regions in the ellipse scatterplot.Further analysis for the two principal components PC1 and PC2,the content of glucose,N-acetylglycoproteins（2.02ppm）,proline（2.06ppm）,ethanol（1.18ppm）,sorbtitol（3.66ppm） and hydroxyproline（2.34ppm） were relatively high in saliva of control group.The content of acetic acid（1.9ppm）,propionate（1.06ppm,2.18ppm） and taurochorate（3.42ppm） were relatively high in saliva of damp-heat stagnating in the spleen group.The content of glutamine（2.14ppm）,sucrose（4.22ppm）,lactate（4.1ppm,4.14ppm） and phenylalanine（3.26ppm） were relatively high in saliva of deficiency of spleen-Qi group.Conclusion：In the physiopathological state of spleen,there are remarkably difference of metabolite profiles of saliva.It reveals the scientific connotation of ＂the spleen con-trolling saliva＂ theory.

关 键 词：脾气虚证 湿热蕴脾证 唾液 代谢组学 

分 类 号：R223[医药卫生—中医基础理论] R228[医药卫生—中医学]