Optimization of Medium Composition for Production of Recombinant Calf Chymosin from Kluyveromyces lactis in Submerged Fermentation  

作　　者：FENG Zhen ZHANG Hongwei ZHANG Yanhe HU Sen JIANG Qian 

机构地区：[1]College of Food Science, Northeast Agricultural University, Harbin 150030, China [2]Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2011年第1期56-62,共7页东北农业大学学报（英文版）

基　　金：Supported by Science and Technology Fund of Heilongjiang Province Education Department (11541018)

摘　　要：Response surface methodology was applied to optimize medium components for production of recombinant calf chymosin by Kluyveromyces lactis GG799.The previous data indicated that the most suitable carbon source,nitrogen source,salt and vitamin were glucose,yeast extract,KH2PO4 and Ca D-Pantothenate,respectively.The concentration of four media components were optimized by using central composite design of response surface methodology.The optimum medium composition for recombinant calf chymosin production was found to contain glucose 29.84 g· L-1,yeast extract 19.85 g·L-1,KH2PO4 0.1 g·L-1 and Ca D-Pantothenate 4.49 mg·L-1.The enzyme activity of recombinant calf chymosin was 722 U· mL-1,which was in an excellent agreement with the predicted value（723 U·mL-1）.The production of recombinant calf chymosin from Kluyveromyces lactis GG799 was effectively increased by response surface methodology.Response surface methodology was applied to optimize medium components for production of recombinant calf chymosin by Kluyveromyces lactis GG799.The previous data indicated that the most suitable carbon source,nitrogen source,salt and vitamin were glucose,yeast extract,KH2PO4 and Ca D-Pantothenate,respectively.The concentration of four media components were optimized by using central composite design of response surface methodology.The optimum medium composition for recombinant calf chymosin production was found to contain glucose 29.84 g· L-1,yeast extract 19.85 g·L-1,KH2PO4 0.1 g·L-1 and Ca D-Pantothenate 4.49 mg·L-1.The enzyme activity of recombinant calf chymosin was 722 U· mL-1,which was in an excellent agreement with the predicted value（723 U·mL-1）.The production of recombinant calf chymosin from Kluyveromyces lactis GG799 was effectively increased by response surface methodology.

关 键 词：chyrnosin Kluyveromyces lactis response surface methodology medium optimization 

分 类 号：TS201.25[轻工技术与工程—食品科学]