脉冲电场促进宫颈癌HeLa细胞摄取光敏剂的实验研究  

Pulsed Electric Fields Enhancing Uptake of Photosensitizer into HeLa Cells:An Experimental Study

作　　者：刘世东[1] 郑飞云[1] 杨孝军[1] 韦伟[1] 

机构地区：[1]温州医学院附属第一医院妇科,浙江省温州市325000

出　　处：《中国全科医学》2011年第9期987-990,共4页Chinese General Practice

基　　金：浙江省自然科学基金(Y206482)

摘　　要：目的探讨脉冲电场对人宫颈癌HeLa细胞摄取光敏剂"二氢卟吩E6"的促进作用。方法以人宫颈癌HeLa细胞为研究对象,通过细胞毒性实验比较单独或联合应用脉冲电场与光动力治疗时细胞的杀伤效应的差异;通过激光共聚焦显微镜半定量与流式细胞术定量观察细胞内荧光物质的强度。结果脉冲电场联合光动力治疗提高了细胞的杀伤效率,强于单纯光动力治疗组;激光共聚焦显微镜半定量观察可见,脉冲电场联合光动力治疗组细胞内的荧光亮度明显高于单纯光动力治疗组;流式细胞术定量检测证实了脉冲电场联合光动力治疗,显著增加HeLa细胞对光敏剂的摄取(P<0.001)。结论脉冲电场显著促进宫颈癌HeLa细胞摄取光敏剂,可有效增加光动力治疗的效率,减少其副作用。Objective To investigate the promotion of pulsed electric fields in enhancement of the uptake of photosensitizer ＂chlorin E6＂ into human cervix cancer HeLa cells. Methods HeLa cells were given with pulsed electric fields or/and photodynamic therapy to compare the difference of cell killing effects by cytotoxicity test;the intensity of fluorescent substances was observed semi-quantitatively by confocal laser scanning microscopy（CLSM） and quantitatively by flow cytometry. Results Pulsed electric fields with photodynamic therapy improved cell killing effects,stronger than single photodynamic therapy.CLSM semi-quantitative analysis showed that fluorescence intensity of pulsed electric field combined with photodynamic therapy was significantly higher than that of single photodynamic therapy.Flow cytometry quantitative analysis showed that pulsed electric field with photodynamic therapy enhanced the uptake of photosensitizer into HeLa cells（P0.001）. Conclusion Pulsed electric field,enhancing significantly the uptake of photosensitizer into HeLa cells,can increase effectively the efficiency of photodynamic therapy and decrease side effects.

关 键 词：脉冲电场 光动力治疗 光敏剂 激光共聚焦显微镜 流式细胞术 

分 类 号：R711.74[医药卫生—妇产科学]