系统性红斑狼疮患者血清多肽的MALDI-TOFMS分析研究  

A proteomic study of serum peptidome patterns of human systemic lupus erythematosus based on MALDI-TOF mass spectrometry analysis

作　　者：文锦丽[1] 戴勇[1] 黄礼玲[1] 胡成效[1] 王琳纤[1] 

机构地区：[1]暨南大学第二临床医学院,广东省深圳市人民医院临床研究中心,深圳518020

出　　处：《中国免疫学杂志》2011年第3期265-268,273,共5页Chinese Journal of Immunology

基　　金：深圳市科技计划项目(200902012)

摘　　要：目的:本实验应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)寻找SLE患者血清中与疾病相关及条件特异的差异性表达多肽,尝试建立SLE的多肽鉴别诊断模型。方法:将SLE患者分为活动期和非活动期两组,以健康志愿者作为正常对照组。应用弱阳离子交换磁珠分离纯化血清样本,MALDI-TOF-MS获取样本生物学信息,建立分类预测模型。结果:成功检测并鉴别出了一系列的差异性多肽,建立了具有高的预测能力和交叉验证能力诊断预测模型。盲法验证该分类模型显示,对活动期SLE组的诊断敏感性达91.7%,非活动期SLE组诊断敏感性达83.3%,对正常对照组的诊断特异性达86.7%。结论:运用多肽指纹图谱法从一个整体的角度进行疾病研究,为更好地理解SLE的发病机制和改进SLE的诊断方法进行了初步的探索。Objective：To find differentially expressed disease-related and condition-specific peptides in sera from patients with SLE by utilization of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALD1-TOF-MS）,and to develop and validate the peptide classification model for diagnosis of SLE.Methods：SLE patients were divided into two groups,active SLE and stable SLE,and healthy donors were as normal control.We performed magnetic beads-based weak cation exchange for sample processing and MALDI-TOF-MS for peptide profiling.ClinProt software 2.1（Bruker Daltonics Company） was used for data analysis and genetic algorithm was modeled for class prediction.Results：A series of significant short peptides were successfully detected and identified.Classification models were developed,and achieved high capability of prediction and cross-validation.Blinded verification of the classification model showed 91.7% sensitivity in active-SLE,83.3% sensitivity in stable-SLE and 86.7% specificity in normal controls.Conclusion：A preliminary work is done for a better understanding of the pathogenesis of SLE and improving diagnosis of SLE from an integrated perspective of peptide mass fingerprinting.

关 键 词：系统性红斑狼疮 蛋白质组学 质谱 分析 

分 类 号：R593.241[医药卫生—内科学]