热休克处理对TNF-α诱导胃腺癌细胞凋亡作用的影响  

Effect of heat shock treatment on the apoptosis of gastric adenocarcinoma cells induced by TNF-α

作　　者：王学哲[1] 张蕴莉[1] 李昆[1] 唐甜甜[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《山东医药》2011年第7期30-31,共2页Shandong Medical Journal

摘　　要：目的探讨热休克处理在肿瘤坏死因子-α(TNF-α)诱导胃腺癌BGC823细胞凋亡过程中的作用,为胃癌的生物学治疗提供依据。方法将体外培养的胃腺癌BGC823指数生长期细胞分为热休克组和对照组,热休克组行热休克处理。两组均分别用0、25、50、100 ng/m1浓度的TNF-α诱导,作用4 h;热休克组行热休克处理。采用TUNEL法和3H-TdR掺入试验检测两组肿瘤细胞凋亡指数(AI)及细胞增殖抑制率(IR)。结果与对照组比较,热休克组AI明显下降(P<0.01);IR明显升高(P<0.01),呈剂量依赖性。结论热休克处理对TNF-α诱导的胃腺癌BGC823细胞凋亡具有抑制作用;此作用与TNF-α呈剂量依赖效应。ObjectiveTo investigate the effect of heat shock treatment the apoptosis of gastric adenocarcinoma cells BGC823 induced by TNF-α.MethodsGastric adenocarcinoma cells cultured in vitro were divided to heat shock group and control group,an then treated by TNF-α of 0,25,50,100 ng/ml for 4 hs.TUNEL and 3H-TdR test were used to examine apoptosis index and DNA synthsis.ResultsCompared with the control group,the apoptosis index of heat shock group decreased significantly（P0.01）;and the cpm significantly increased（P0.01）,which showed dose-depended manner.ConclusionsHeat shock treatment can inhibit the apoptosis of gastric adenocarcinoma cells induced by TNF-α in vitro;this effect showed dose-depended manner.

关 键 词：热休克处理 热休克蛋白 胃腺癌 细胞凋亡 肿瘤坏死因子-Α 

分 类 号：R735.2[医药卫生—肿瘤]