Tca8113肿瘤微环境诱导小鼠髓源性树突状细胞免疫耐受性的研究  

Immune Tolerance of bone marrow-derived dendritic cell induced by Tumor Microenvironment of tca8113

作　　者：童熹[1] 李勇[1] 李显[1] 王启明[1] 王涛[1] 

机构地区：[1]重庆医科大学口腔医学院口腔颌面外科,400015

出　　处：《健康必读（下）》2011年第3期19-21,共3页

基　　金：重庆市科委自然科学基金资助课题（CSTC2004BB5117）重庆市卫生局科研基金资助课题计划（07-2-178）;重庆市教委科学技术研究项目基金资助课题（KJ090326）

摘　　要：目的：tca8113肿瘤微环境诱导骨髓CD34＋细胞分化为免疫耐受树突状细胞体外实验研究。方法：利用无菌小鼠长骨骨髓细胞，对照组在常规重纽小鼠粒细胞一巨噬细胞集落刺激因子（rmGM-CSF）和重组小鼠白介素-4（rmIL-4）培养液中培养，诱导分化为DCs，实验组在培养样体系中再加入舌鳞癌细胞株培养上清液，对照组不加任何干预因素。培养六天后加入（LPS）观察其对外源性刺激的反应，流式细胞术检测DCs表型CD86、CD11C、MHC—II表达，混合淋巴细胞增殖实验比较其在体外刺激T淋巴细胞增殖能力。结果：tca8113肿瘤微环境不能抑制DCs成熟；tca8113-DC和LPS＋tea8113-DCMCH—II表达高于Dc和LPS＋DC，p〈0．05；tca8113-[mDC在LPS刺激后仍能保持未成熟，MCH-II表达无明显变化p〉0．05；tca8113-DC刺激T淋巴细胞增殖的能力弱于对照组，p〈0．05。结论：肿瘤微环境诱导小鼠髓源性树突状细胞产生免疫耐受性，可能是肿瘤细胞免疫逃逸机制的关键。Objective： To investigate immune tolerance of bone marrow-derived dendritic cell induced by tumor microenvironment of tca8113. Materials ＆ Methods： Culture supernatural of Tca8113 was collectd in vitro and then cooperate with rmgranulocyte-macrophage colony-stimulating（rmGM-CSF） and rminterleukin-4（rmlL-4） to induce DCs derived from C57BL/6 bone marrow, after 6 days DCs were stimulated by LPS for 24h.Tbese DCs＇ phenotype of cells was analyzed by flow cytometry, their ability to stimulate T lymphocyte proliferation were compared by mixed lymphocyte reaction iu vitro. Kesults： Tumor microeuvironment do not inhibit the mature of DCs; expression of MCH- II oftca8113-DC and LPS＋tca8113-DC is more then DC and LPS＋DC p〈0.05; tca8113-imDC restain status of immature after stimulated by LPS; the ability of tca8113-DC and LPS＋tca8113-DC to stimulate T lymphocyte proliferation was distinctly lower than that of DC and LPS＋DC. Conclusion： Immune tolerance of bone marrow-derived dendritic cell induced by tumor microenvironment oftca8113.

关 键 词：肿瘤微环境 树突状细胞 免疫耐受 免疫抑制因子 

分 类 号：R73[医药卫生—肿瘤]