哈夫尼菌降解毒死蜱条件的优化及动力学模型建立  

Optimization of Hafnia sp.to Chlorpyrifos Degradation Conditions and a Kinetics Model

作　　者：陶玉贵[1] 王其进[1] 谷浩[1] 倪正[1] 秦昌云[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《农业环境科学学报》2011年第3期449-454,共6页Journal of Agro-Environment Science

基　　金：安徽省科技攻关计划面上项目(08010302169)

摘　　要：利用响应面法优化哈夫尼菌降解毒死蜱的条件,并建立了降解模型。采用Box-Behnken设计试验,以响应面法进行优化,结果表明,哈夫尼菌降解毒死蜱的最优条件为:蔗糖浓度0.31%,毒死蜱初浓度51.33mg·L-1,培养温度30.7℃,在该条件下理论预测毒死蜱降解率可达74.7%。通过假设和验证,得出哈夫尼菌降解毒死蜱为一级动力学模型,哈夫尼菌能水解毒死蜱P-O键,属一级酶促反应。The objective of this study was to determine the optimum conditions for the biodegradation of chlorpyrifos by Hafnia sp.,and to establish a kinetic model to simulate the degradation process.Response surface methodology（RSM） based on Box-Bohnken experimental design was employed to determine the optimum conditions.Results indicated that the optimum conditions for chlorpyrifos degradation based on response surface and contour plots were as follows：concentration of sucrose,0.31%（w）;initial concentration of chlorpyrifos,51.33 mg·L-1;culture temperature,30.7 ℃.In addition,the predicted value of chlorpyrifos degradation rate under the optimum conditions was 74.7%,which was in good agreement with the experimental result：under the optimum conditions,the degradation rate of chlorpyrifos was 72.2% after 72 hour＇s culture.Moreover,according to hypothetical kinetic model for chlopyrifos degradation,the kinetics for chlorpyrifos degradation could be described by a first-order equation,which is similar to an enzyme-catalyzed reaction.A possible mechanism for the degradation of chlorpyrifos by Hafnia sp was that this microorganism had produced an enzyme that can cleave the P-O bond of chlorpyrifos.

关 键 词：哈夫尼菌 毒死蜱 响应面 动力学 

分 类 号：X172[环境科学与工程—环境科学]