IL-2基因修饰增强白血病抗原冲击的树突状细胞诱导的抗肿瘤免疫反应  

Induction of potent antitumor immune response of leukemia antigen pulsed dendritic cells by IL 2 gene modification

作　　者：唐华[1] 曹雪涛[2] 朱学军[2] 张明徽[2] 袁正隆 于益芝[2] 章卫平[2] 

机构地区：[1]河南省人民医院生物治疗中心 [2]第二军医大学免疫学教研室,上海200433

出　　处：《中华血液学杂志》1999年第11期573-576,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：Objective To investigate whether adenovirus mediated IL 2 gene modification can improve antitumor immunity of the tumor antigen pulsed dendritic cells(DC) in vivo. Methods DC were generated from bone marrow, then, pulsed with FBL 3 erythroleukemia cell lysates and transfected with IL 2 gene simultaneously. IL 2 secretion and T cell stimulating activity of the DC were detected. The number and cell subsets of draining lymph nodes, cytotoxic lymphocyte(CTL) activity ,and the increase of protective effect for the mice s.c vaccinated with DC were observed. Results IL 2 gene modified, antigen pulsed DC could secrete high level of IL 2 in vitro, stimulate proliferation of syngeneic T cells markedly. DC vaccine could increase the number of draining lymph node and induce CTL activity, which directed to FBL 3, more significantly. After the DC vaccination, the survival time of the mice challenged with wild type FBL 3 cells were prolonged. Conclusion Antitumor immune response could be induced more potently by vaccination with IL 2 gene modified, antigen pulsed DC.Objective To investigate whether adenovirus mediated IL 2 gene modification can improve antitumor immunity of the tumor antigen pulsed dendritic cells(DC) in vivo. Methods DC were generated from bone marrow, then, pulsed with FBL 3 erythroleukemia cell lysates and transfected with IL 2 gene simultaneously. IL 2 secretion and T cell stimulating activity of the DC were detected. The number and cell subsets of draining lymph nodes, cytotoxic lymphocyte(CTL) activity ,and the increase of protective effect for the mice s.c vaccinated with DC were observed. Results IL 2 gene modified, antigen pulsed DC could secrete high level of IL 2 in vitro, stimulate proliferation of syngeneic T cells markedly. DC vaccine could increase the number of draining lymph node and induce CTL activity, which directed to FBL 3, more significantly. After the DC vaccination, the survival time of the mice challenged with wild type FBL 3 cells were prolonged. Conclusion Antitumor immune response could be induced more potently by vaccination with IL 2 gene modified, antigen pulsed DC.

关 键 词：白血病 白细胞介素2 基因治疗 T细胞 

分 类 号：R733.705[医药卫生—肿瘤]